| Objective: To study the changes of p-Akt(Ser473) expression and Puerarin's effects in the ischemic penumbra cortex after MCAO in rats, and investigate the effects of this changes on cerebral ischemia injury and Puerarin's interference mechanism. Methods: Sprague-Dawley male rats(n=60) were randomly divided into 5 groups(n=12): sham operate group, ischemic group, LY294002 intervention group, puerarin intervention group, puerarin and LY294002 intervention group. SD rat permanent MCAO models were performed by the improved traditional Zea Longa'method. All rats were executed after neurological deficit were monitored 12h after operate, Then every group randomly 6 brain for HE ,TUNEL and immunohistochemistry stain, to observe the apoptosis and the expression of p-Akt(Ser473) protein; The other 6 brain sections were stained by TTC for monitor the area of infarct. Results: 1. The expression of p-Akt(Ser473) marked decrease (P<0.05), and TUNEL-positive cells increase and neurological functional deficit score increase at 12h after MCAO in Ischemic group (P<0.05). 2. There's no changes on the p-Akt(Ser473) expression, TUNEL-positive cells and neurological functional deficit score at LY294002 intervention group 12h afer MCAO(P<0.05). 3. The p-Akt(Ser473) expression in the ischemic penumbra cortex after MCAO significantly increase in puerarin intervention group (P<0.05), and TUNEL-positive cells and neurological functional deficit score are decreased significantly too(P<0.05); Moreover, puerarin's effects on the expression of p-Akt(Ser473) and inhibiting cells Apoptosis improving neurological functional were interferenced significantly by LY294002. Conclusion: The changes of p-Akt(Ser473) expression play a very important role for the ischemic tolerance after MCAO; Puerarin can increase the expression of p-Akt(Ser473) and reduce the number of apoptotic cells, improving neurological functional; The mechanisms relate to maintaining the signal transduction of PI3K/Akt activating.
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