Research Of The San Ao-tang And Its Serial Decoctions' Action, Mechanism Of Action And Material Basis In The Occupational Asthma Rat Model Sensitized By TMA

ObjectiveTo constructe the model of occupational asthma on rat and research the effect and material baseof San Ao-tang and its Serial Decoctions based on the model.MethodsSensitize the occupational asthma rats with the low-molecular-weight chemical.According to theethological sign, the cytologic examination of the blood and BALF, the pathological examinationof lungtissue, provocative test of Ach in vitro rat airway smooth muscle as judgement index,comments on dose-effect relationship of San Ao-tang and its Serial Decoctions,ethyl acetateextracts and the dosage.Results1 .Succeed to make the occupational asthma rat model,which significantly show short of breath,abdominal curl and breast reduction. Percentages of EOS cells and concentration in the bloodand BLAF all ascend more or less. the pathological examination of lungtissue show thatepithelium of bronchus had various degrees degeneration and necrosis.the inflammatory exudatesuch as eosinophilic granulocyte, monocyte and lymphocyte abound in the bronchial lumen.thethickened bronchial loops,and the narrowing of the lumen be observed inflammatory reaction.2.San Ao-tang and its Serial Decoctions have a significant improvement in the occupationalasthma rat sensitized by TMA varying degrees. The most obvious example is the improvementof San Ao-tang Decoction III in indexes.3.The effect dose-effect relationship of San Ao-tang Decoction III to the occupational asthmarat sensitized by TMA present obviously positive correlation.The effect of the Megadose is the strongest, And middle dose takes second place, small dose isthe Weakest.4.The effect dose-effect relationship of San Ao-tang Decoction III ethyl acetate extracts to theoccupational asthma rat sensitized by TMA present obviously positive correlation.The effect ofthe Megadose is the strongest, And middle dose takes second place ,small dose is the Weakest.Conclusion1.Succeed to have constructed the model of the occupational asthma rat according to theethological sign, the cytologic examination of the blood and BALF, the pathological examinationof lungtissue.2. The most obvious example in decoctions is the improvement of San Ao-tang DecoctionIII,with significantly dose-effect relationship. 3. San Ao-tang Decoction III ethyl acetate extracts has a significantly effect in indexes, with significantly dose-effect relationship.