| Objective:This dissertation was aimed to search some blood components and direct material generated from model rats which stomach filled Sheng Mai San combining with effect to cure and prevent alzheimer’s disease (AD) based on the "Chinese medicine serum drug chemical theory".Methods:Rat model was successfully established by the method of fill the stomach aluminium trichloride, intraperitoneal injection of D-galactose in order to explore Intervention effect of the rats use Sheng Mai San,evaluate model through the observation of pathological and behavior in rats. Then find Blood transitional components and direct material generated from model rats which stomach filled Sheng Mai San. Finally clarify its Pharmacological effects.Results:1. Establishment and evaluation of alzheimer’s disease model ratsThis experiment adopts the filled aluminium trichloride, intraperitoneal injection of D-galactose method to establish model rats. Through on the histopathological observation of model group showed that rats’CA3 area of hippocampal damaged obvious, neurons decrease and atrophy significantly. Compared with blank group, model group neurons reduced, cytoplasmic yellowing, a significant reduction in the average grey value. Its all showed that model group’A β1-40 (senile pigment) increased. In combination with behavior observed, model rats’ escape latent period prolong than model group obviously (P<0.01), and model group compared with the blank group in quadrant, stay time significantly reduce in the effective area. After a number of platform also decreased significantly (P<0.01).2. intervention of Sheng Mai San effective on alzheimer’s diseasePathological showed that the administrated group rats compared with model group, the former’pyramidal cellular morphology of cerebral cortex neuron were improved. The main performance is pyramidal cells became orderly arrangement, obvious nucleus, uniform color, shallow blue purple, and the number of neurons increased significantly. Administrated group rats’CA3 area of Hippocampal also gradually getting better too. This effect is especially high dose is more significant, and the Pathological as previously mentioned are basically improve.3. Basic research of Sheng Mai San effect on the AD rat modelSerum sample data which collected from administrated rats did PCA analysis, which using UPLC Synapt G2-Si, combined with MS and MS/MS data which selected ion. Meanwhile refer to vitro chemical composition information of Sheng Mai San identified 12 blood composition, respectively:20(S)-Ginsenoside Rhl,20(R)-Ginsenoside Rhl,Ginsenoside Rk3,Ginsenoside Rh4,Schisandrin,Gominsin D,Schisandrol B,Schisantherin A,Schisantherin B,Deoxyschisandrin, γ-Schisandrin and Schisandrin B. After apply Metabolynx analysis AD rat’s blood components which ate Sheng Mai San, which found that Schisandrin, Deoxyschisandrin common metabolic produced three kinds of metabolites, respectively:M3, Gominsin, M12. Schisandrol B metabolic produced seven kinds of metabolites, respectively:Ml, M2, M4,7,8-dihydroxy-2-demethyl schizandrin, M6, M8, M11. Schisandrin B metabolic produced seven kinds of metabolites, respectively:M4, M6, Schisandrin, M8, M9, M11, M13. Schisandrol B and Schisandrin B common produced four kinds of metabolites, respectively:M4, M6, M8, M11.Conclusion:This experiment preliminary sure 12 blood components,13 metabolites based on the Chinese medicine serum drug chemical theory, through the method which using UPLC Synapt G2-Si intervene AD rat’ s blood sample, PCA analysis and Metabolynx data processing techniques. This dissertation lays the foundation for the further study of Sheng Mai San effective on alzheimer’s disease. As well as give the reference to others who research composition analysis of outside and inside body. |
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