Research On Concentration Of Serum And Urine Cysteinyl Leukotriene In Infants With Asthmatic Diseases

Objective:To detect serum of cysteinyl leukotriene B₄(LTB₄)and urine of cysteinyl leukotriene E₄(LTE₄)concentration in infants with asthmatic diseases,and to explore the relationship with asthmatic diseases,pneumonia mycoplasma(MP),respiratory syncytial virus(RSV),and infants atopic status.Methods:There were 30 infants with asthmatic diseases in the study group,and 20 infants with non-asthmatic diseases but with respiratory tract infection in the control group.Serum and urine samples were collected. Concentration of LTB₄ and LTE₄ in those samples were measured by enzyme linked immunosorbent assay(ELISA).Serum Mp-IgM and RSV-IgM were also analyzed by ELISA.The results were analyzed by SPSS 13.0.Results:1.MP-IgM positive 4 cases(13.3%),RSV-IgM positive 12 cases(40%), MP-IgM and RSV-IgM both positive 1 case(3.3%).2.Analysis of LTB₄ concentration:That in asthmatic group was higher than that in non-asthmatic group(262.06±70.55 vs 197.10±45.43,P＜0.05).That in MP-IgM positive group was higher than that of MP-IgM negative group(348.48±107.18 vs 248.76±54.91,P＜0.05),but RSV-IgM positive group and RSV-IgM negative group had no significant difference(268.07±36.41 vs 259.48±81.63,P＞0.05).LTB₄ level in infants in asthmatic diseases group with atopic background group was higher than that of non-atopic status infants (323.04±87.43 vs 235.92±42.04,P＜0.05).3.Analysis of LTE₄ concentration:Concentration of LTE₄ in acute asthma period was higher than that of non-asthmatic group(247.17±83.40 vs 128.43±46.150,P＜0.05),with the ease of asthma,the concentration of LTE₄ decreased significantly,in asthma remission,the concentration of LTE₄ was lower than that of acute asthma period(177.44±43.86 vs 247.17±83.40,P＜0.05),but was still higher than that of non-asthmatic group(177.44±43.86 vs 128.43±46.15,P＜0.05).In the asthmatic group,MP-IgM positive group was higher than that of MP-IgM negative group(409.92±100.47 vs 244.44±49.59,P＜0.05),but there were no significant difference between RSV-IgM positive group and RSV-IgM negative group(262.06±45.74 vs 243.65±98.02,P＞0.05),LTE₄ level in infants in asthmatic diseases group with atopic background group was higher than that of non-atopic status infants (336.76±89.64 vs 211.63±49.05,P＜0.05).4.Serum LTB₄ concentration and urine LTE₄ concentration was positively correlated(γ=0.742,P＜0.05).Conclusion:Concentration of cysteinyl leukotriene B₄ and cysteinyl leukotriene E₄ in infants with asthmatic diseases maintained a high level. Leukotrienes is one of the major factors that led to the infants wheezing episode.