Expression Of IL-6,IL-18 In Matenal Serum,Umblical Code Serum And Fetal Membranes In Pregnant Woman With Premature Rupture Of Membranes

Objective:Through detect the expression of IL-6,IL-18 in maternal serum,umbilical code serum and fetal membrane in pregnant woman with premature rupture of membranes,to approach the relationship between them and premature rupture of membranes,the feasibility to anticipate chorioamnionitis.Methods:Women with premature rupture of membranes and normal women were studied,the expression of IL-6,IL-18 in maternal serum and umbilical code serum were measured by enzyme-linked immunosorbent assay.The expression of IL-6,IL-18 in fetal membrane were detected by immunohistochemistry.Results:1.The lever of IL-6 in maternal serum and umbilical code serum in TPROM was(25.71±6.46,24.35±5.12) ng/ml,the lever of IL-6 in maternal serum and umbilical code serum in PPROM was(28.47±6.9,26.24±5.54) ng/ml,the two group had no significant difference (P＞0.05),but both significantly higher than that in the control group (22.88±6.52,22.77±5.55) ng/ml(P＜0.05).2.The lever of IL-18 in maternal serum and umbilical code serum in TPROM was(32.74±9.64, 27.74±6.64) ng/ml,the lever of IL-18 in maternal serum and umbilical code serum in PPROM is(35.64±11.96,31.11±10.63) ng/ml,the two group had no significant difference(P＞0.05),both significantly higher than that in the control group(21.72±7.41,23.32±5.11) ng/ml(P＜0.05). 3.With the grade of chorioamnionitis raised,the expression of IL-6 and IL-18 in maternal serum,umbilical code serum,fetal membranes were set up(P＜0.05).There was positive correlation between chorioamnionitis and the expression of IL-6,IL-18 protein,R is 0.565,0.513.4.The level of IL-6,IL-18 in maternal serum(34.95±11.12),(51.54±16.6) ng/ml whose got neonatal asphyxia is higher than the normal neonate group (24.63±8.18),(27.69±8.34) ng/ml.But in neonatal asphyxia,the level of IL-6,IL-18 in maternal serum,umbilical code serum between chorioamnionitis and nonchorioamnionitis are not different.5.According to the method of Youden Index,when the level of IL-18 in maternal serum was divided 23.09 ng/ml,the sensitivity to predict chorioamnionitis was 88%, the specificity was 82%.;When the level of IL-6 in maternal serum was divided 24.58 ng/ml,the sensitivity to predict chorioamnionitis is 72%, the specificity was 68%.Conclusion.1.IL-6,IL-18 are concerned with PROM and chorioamnionitis.They are valuable clinical index for identifying the chorioamnionitis in patients with PROM.2.There is a positive correlation between level of IL-6,IL-18 in maternal serum and umbilical code serum.They are maybe associated with asphyxia of newborn.