Effect Of P38 MAPK Specific Inhibitor SB203580 In Rat Nonbacterial Chronic Prostatitis

Part 1 Expression of p38 MAPK in Rat Nonbacterial Chronic Prostatitis ModelObjective: To approach the expression of p38 MAPK in rat CNP model, and it's relationship of tumour necrosis factor-α( TNF-α), matrix metalloproteinase-9(MMP-9), cyclooxygenase-2(COX-2), phosphorylated p38(p-p38) in CNP pathogenesy.Methods: The adult maleness SD rats model was set up by four weeks subcutaneously hypodermic injection with benzestrofol after castration, control group without pretreat. H.E. staining pathology change of the two groupes of prostatae tissue were observed under light microscope, protein expression of TNF-α, MMP-9, COX-2, p-p38 were detected by immuno- histochemistry, mRNA level of p38 was checked by RT-PCR.Results: No inflammatory reaction was found in prostate tissue of control group, by contrast, model group had obvious inflammatory reaction. The protein expression of TNF-α, MMP-9, COX-2, p-p38 in control group was low, but higher expression in model group. There was statistical significance between two groups (p<0.05). The p38 mRNA level of control group was only 37.3% of model group.Conclusion: p38 MAPK signal transduction pathway was actived in CNP, and the expression of TNF-α, MMP-9, COX-2 may be regulated by p38 MAPK, suggesting a new pathway for the study and therapy of CNP.Part 2 Effect of p38 MAPK Specific Inhibitor SB203580 in Rat Nonbacterial Chronic ProstatitisObjective:To approach the role of SB203580, an specific inhibitor of mitogen activated protein kinase P38, on rat chronic nonbacteral prostatitis and it's effect on the expression of TNF-α, MMP-9, COX-2.Methods: Adult male SD rats were randomly divided into three groups, model and treated groups were castrated and hypodermically injected with benzogynestry for 4 weeks, also treated group were injected with SB203580 intraperitoneally every two days, then set up control group without any treatment. H.E. staining pathology of prostate gland tissue was checked under light microscope, the expression of TNF-α, MMP-9, COX-2, p-p38 each group were checked by immunohistochemistry and western blot respectively, and the mRNA level of p-p38 was checked by RT-PCR.Results:No inflammatory reaction was observed in control group, but obvious inflammatory reaction in model group, and inflammatory reaction relieved after treated with SB203580. The protein expression of TNF-α, MMP-9, COX-2, p-p38 were low in control group, but high in model group. After treated with SB203580, all the four genes's expression decreased significantly and can be compared to control group. Meanwhile, the expression trend of p-p38 was relative to that of TNF-α, MMP-9, COX-2 respectively. No statistically significant difference of P38 mRNA expression was found between control and SB203580 treated groups, but the mRNA expression of p38 in model group was higher than other two groups.Conclusion:In CNP rat model, SB203580, the specific inhibitor of mitogen activated protein kinase P38, maybe improve prostatic inflammation through decreasing the expression of cytokines TNF-α, MMP-9, COX-2, providing new evidence for therapy of CNP.