The Study Of Relationship Between MPO Gene-463G>A And -638C>A Polymorphisms And Stroke

Objective:To investigate the association between the MPO -463G＞A and -638C＞A polymorphisms and stroke,hypertension, atherosclerosis and also the metabolism of the blood lipids.Methods:The genotypes of 273 patients with atherosclerotic cerebral infarction,205 patients with primary cerebral hemorrhage and 213 sex-and-age-matched health controls were detected by ASP-PCR. Serum lipid concentrations were determined by enzymatic analytical chemistry.The HWE software was used to check the goodness of fit of Hardy-Weinberg balance of the genotypes.All date was analyzed by SPSS 13.0.Result:(1)-463G＞A and -638C＞A of MPO polymorphisms exist in Chinese people of Hunan area,with the allele frequencies 0.761/0.239 for -463G＞A and 0.845/0.155 for -638C＞A respectively.No significant differences were observed in genotypes and allele frequencies between stroke patients and controls for MPO -463G＞A,-638C＞A(P＞0.05).Logistic regression analysis suggested that neither any genotypes no any alleles could increase the risk of onset of stroke(P＞0.05).(2)The difference of the frequency of MPO -463G＞A,-638C＞A genotypes and alleles between the hypertension subgroup and the nohypertension subgroup of the CI and CH group were not significant(P ＞0.05).The similar result was observed between the family history and no family history of CI and CH group(P＞0.05).(3) The mean serum level of HDL-c of -463G＞A GA carriers and AA carriers was higher than GG carriers in CI group(P＜0.05);The mean serum level of LDL-c of -463G＞A GA+AA carriers and AA carriers was lower than GG carriers in CI and CH group(P＜0.05).(4)The difference of the frequency of MPO -638C＞A genotypes and alleles between the CI+DM subgroup and the controls was significant(P＜0.05).(5)The difference of the frequency of MPO -463G＞A genotypes and alleles between the CI+AP subgroup and the CI+NAP subgroup was significant(P＜0.05);The difference of the frequency of MPO -463G＞A genotypes and alleles between the CH+AP subgroup and the CH+NAP subgroup was significant(P＜0.05).(6)The incidence rate of DM of -638CA+AA and -638CC carriers had significant difference in -463 GG subgroup;The mean serum level of LDL-c of -638CA+AA carriers was higher than CC carriers in -463 GG subgroup(P＜0.05).Conclusion:(1) There is probably no association between the MPO -463G＞A and -638C＞A polymorphism and CI or CH in han population in Hunan province. (2)There is probably some association between the MPO -463G＞A polymorphism and the carotid artery atherosclerosis plaque in han population in Hunan province.(3)There is probably some association between the MPO -463G＞A and -638C＞A polymorphism and the metabolism of HDL-c and LDL-c in Chinese han population.A allele might be an important factor of affecting the serum levels of HDL-c and LDL-c.(4)MPO -463G＞A and -638C＞A polymorphism have a possible interaction in their effects on the presence of LDL concentrations and DM.