The Dose-effect Of Lycopene On Regulating Blood Lipids In Rats

Objective:In order to study the dose-effect on lycopene regulation of plasma lipid in hyperlipidemia rats;to provide the evidence for the exploitation of lycopene and utilizion in body and research further.Methord:According to the level of serum total cholesterol and weight, Sixty-four adult male SD rats were divided randomly into eight groups: normal control,fed by normal feed;hyperlipidemia group:fed by CCT diet; positive control group:fed by CCT diet plus 10 mg·kg·b/w·d fluvastatin sodium;dose of lycopene groups:fed by CCT diet plus 4.4,11,22,44,110 mg·kg·b/w·d lycopene;intragastric administration.The level of serum total cholesterol,total triglyceride,low-density lipoprotein cholesterol, high-density lipoprotein cholesterol,SOD,and MDA were measured.At the end of the reasrch,the rats were narcosisd by tuina.Morphologic aorta atherosclerosis was observed by HE staining methods.Select morph,liver, kidney,testicle and weight them to compute the index number.Result:1.Fed by CCT diet one week,groups B rats of serum lipids were higher than group A(P＜0.05).As the experimental session extended,the serum TC,TG,LDL-C of group B were become higher gradually,at the end of the experiment,TC,TG,LDL-C were 13.56,4.51,6.65 times than the start of the group B(P＜0.05);the pathological section showed that endomembrane was thicked obviously,vessel wall oncoidesd,foam cells heaped up,some reached tunica media,the structure lamina superficialis of tunica media disordered.2.By the multivariate analysis of variance of repeated measure,the data showed that different doses of lycopene at different experiment stage had different degree of regulation to TC(F=5265.297,P=0.000).By curue fitting analysis,the result of week 2(R²=0.649,P=0.000) and 3(R²=0.634,P=0.000) showd that as the increasing doses of lycopene,they could reduce the role of serum TC,and showd the obviously relationship of square plot;By the multivariate analysis of variance of repeated measure,the data showed that different doses of lycopene at different experiment stage had different degree of regulation to TG(F=391.972,P=0.000).By curue fitting analysis,the result of week 2(R²=0.900,P=0.000) and 3(R²=0.926,P=0.000) showd that as the increasing doses of lycopene,they could reduce the role of serum TG,and showd the obviously relationship of square plot;By the multivariate analysis of variance of repeated measure,the data showed that different doses of lycopene at different experiment stage had different degree of regulation to LDL-C(F=2645.165,P=0.000).By curue fitting analysis,the result of week 2(R²=0.463,P=0.000)and 3(R²=0.717,P=0.000)showd that as the increasing doses of lycopene,they could reduce the role of serum LDL-C,and showd the obviously relationship of square plot;There were no significant effect to HDL-C on any dose of Lycopene(P＞0.05).The dose of lycopene in the 44,110 mg·kg·b/w·d were the same as group C:the bulk of endomemb-rane was integrity,foam cells disappeared,there was not fibro-plasia.3.By the multivariate analysis of variance of repeated measure,the data showed that different doses of lycopene at different experiment stage had different degree of regulation to SOD(F=7014.837,P=0.000),By curue fitting analysis,the result of week 2(R²=0.876,P=0.000) and 3(R²=0.946, P=0.000) showd that as the increasing doses of lycopene,they could increase the role of serum SOD,and showd the obviously relationship of square plot; By the multivariate analysis of variance of repeated measure,the data showed that different doses of lycopene at different experiment stage had different degree of regulation to MDA(F=1844.478,P=0.000),By curue fitting analysis,the result of week 2(R²=0.870,P=0.000) and 3(R²=0.876,P=0.000) showd that as the increasing doses of lycopene,they could reduce the role of serum SOD,and showd the obviously relationship of square plot.Conclusion:1.By the methord of Deepa,fed by CCT diet one week,the rats became hyperlipemia,the methord was easy and quickly,at the end of the experiment groups B hepathological section showed that endomembrane was thicked obviously,vessel wall oncoidesd,foam cells heaped up,some reached tunica media,the structure lamina superficialis of tunica media disordered.2.Between the scope of 11～44 mg·kg bw^-1·d^-1,lycopene reduced TC showd obviously dose-effect;Between the scope of 4.4～22 mg·kg bw^-1·d^-1, lycopene reduced TG showd obviously dose-effect;Between the scope of 4.4～44 mg·kg bw^-1·d^-1,lycopene reduced LDL-C showd obviously dose-effect;3.Between the scope of 4.4～44mg·kg bw^-1·d^-1,lycopene reduced MDA, increased SOD showd obviously dose-effect.