Expression And Significance Of HPV 16 E6,E7 Proteins And Telomerase In The Development Of Cervical Intraepithelial Neoplasia

Objective: To investigate the expression of HPV 16 E6,HPV 16 E7 proteins and telomerase in cervical carcinoma and cervical intraepithelial neoplasia, to explore the relationship between them and the malignant progression of cervical precancerous lesions and to search a molecular marker which can predict the outcome of the vesting of cervical precancerous lesions.Methods: The expression of HPV 16 E6,HPV 16 E7 proteins and telomerase in 30 cases of invasive cell carcinomas(ICC), 30 cases of cervical intraepithelial neoplasia(CIN)Ⅰ,30 cases of CINⅡ, 30 cases of CINⅢand 10 cases of normal cervical tissues were detected using Elivision immunohistochemistry. The data of this study was analysed usingχ2 test, Fisher exact propability, strength grade data conversion using non-parametric rank tests (Kruskal-Walls H test),and correlation using Spearman rank correlation.Results: 1. The expression of HPV16E6 protein in cervical tissue was 20.0%,35.0%,70.0% and 86.7% in normal cervical, CINⅠ-Ⅱ, CINⅢ, and ICC tissues respectively. The trend was gradually increasing. The expression intensity in ICC was distinctively higher than other kinds of tissues (P<0.05, respectively). The expression intensity in CINⅢwas significantly higher than that of CINⅠ-Ⅱcervical tissues and control (P<0.05, respectively) , but there was of no significant difference between CINⅠ-Ⅱand control(P>0.05).2.The expression of HPV16E7 protein in cervical tissue was 30.0%,63.3%,76.7% and 96.7% in normal cervical, CINⅠ-Ⅱ,CINⅢ, and ICC tissues respectively. The trend was gradually increasing. The expression intensity in ICC was distinctively higher than other kinds of tissues (P<0.05, respectively). The expression intensity in CINⅢwas significantly higher than that of CINⅠ-Ⅱcervical tissues and control (P<0.05, respectively) .And the expression intensity in CINⅠ-Ⅱwas significantly higher than that of control (P<0.05) .3.The expression of telomerase in cervical tissue was 30.0%,41.7%,70.0% and 93.3% in normal cervical, CINⅠ-Ⅱ, CINⅢ, and ICC tissues respectively. The trend was gradually increasing. The expression intensity in ICC was distinctively higher than other kinds of tissues (P<0.05, respectively). The expression intensity in CINⅢwas significantly higher than that of CINⅠ-Ⅱcervical tissues and control (P<0.05, respectively) , but there was of no significant difference between CINⅠ-Ⅱand control(P>0.05).4. The correlation between HPV16E6,HPV16E7 proteins and telomerase in cervical affection tissues: the relationship between HPV16E6 protein and HPV16E7 protein was positively correlated in cervicitis and cervical cancer(rs =0.272,P<0.05);the relationship between HPV16E6 protein and telomerase was positively correlated in cervicitis and cervical cancer(rs =0.279,P<0.05);the relationship between HPV16E7 protein and telomerase was positively correlated in cervicitis and cervical cancer(rs =0.376,P<0.05).Conclusion: 1. The positive expression rate and intensity of HPV16E6,E7 proteins and telomerase was gradually increasing with the degree of CIN lesions.2.The expression of HPV16E6,E7 proteins and telomerase is positively correlated in the process of cervical canceration.3. HPV16E6,E7 proteins and telomerase may be treated as prognostic factors of CIN, and further study needs to be done .