| Objective:To observe the expressions of HMGI/Y and Jun-B proteins in gastric carcinoma, and to evaluate their possible effect in cancerogenesis and development of gastric carcinoma.Methods:126 patients enrolled into our study, which come from our hospital from the January 2008 to May 2008, received endoscopy, and diagnosed by gastric tissue pathology. There were 20 cases with normal gastric mucosa, 15 cases with intestinal metaplasia, 16 cases with intraepithelial neoplasia(8 of them were taken from pathology department of our hospital),, and 75 cases with gastric cancer. There were 31 cases with well-moderately differentiated, 44 cases with poorly differentiated in 75 cases with gastric cancer. The 31 gastric cancer located in antrum or gastric angle, 44 gastric cancer located in fundus, cardia or gastric body. The size of 25 gastric cancer was less than 3.0cm, but that of 50 more than 3.0cm. There were 6 cases less than 30 years old, 44 cases between from 30 to 60 years old, 25 cases more than 60 years old in 75 patients with gastric cancer (52 male, 23 female). 49 of 75 cases with gastric cancer accepted surgical treatment in our hospital. Cancer tissue of 23 cases limited in mucosa or submucosa(19 of them were taken from pathology department of our hospital), and cancer tissue of 26 cases infiltrated muscular layer after operation. 27 cases accepted operation had lymph node metastasis, 22 no lymph node metastasis. 25 cases accepted operation were staged in TNM I to II period, 24 cases in TNM III to VI period. The expressions of HMGI/Y and Jun-B in all gastric tissues were detected by immunohistochemistry method. The relationship between the expression of HMGI/Y and Jun-B with clinic pathological features was analyzed.Results:â‘ The positive expression rates of HMGI/Y in normal gastric mucosa, intestinal metaplasia, intraepithelial neoplasia and gastric cancer tissues were 25%, 60 %, 75%, and 88.0% respectively. The more malignant the gastric tissue was, the higher the positive expression rate of HMGI/Y was (P<0.05). The expression intensity of HMGI/Y in gastric cancer was higher than other kinds of tissues (P<0.05), and that in intraepithelial neoplasia was higher than normal gastric tissues (P<0.05), but there was no significant difference between intestinal metaplasia and intraepithelial neoplasia, or between intestinal metaplasia and normal gastric tissues (P>0.05). The HMGI/Y expression intensity in patients with tumor infiltrating muscular layer, having lymph node metastasis, or staged in TNM III to IV period, was significant higher than that with tumor limiting in mucosa and submucosa, having no lymph node metastasis, or staging in TNM I to II period respectively (P<0.05). However, the HMGI/Y expression intensity had no significant difference in different differentiation degree,different cancer region, different tumor sizes, different age, or different sex (P> 0.05).â‘¡The positive expression rates of Jun B in normal gastric mucosa, intestinal metaplasia, intraepithelial neoplasia and gastric cancer tissues were 95%, 93.3%, 87.5%, and 74.7% respectively. The more malignant the gastric tissue was, the lower the positive expression rate of Jun B was. There was significance among positive expression rates of normal gastric mucosa, precancerous tissues, and cancer tissues (P<0.05).The expression intensity of Jun B in gastric cancer was lower than in noaml gastric tissues and intestinal metaplasia tissues (P<0.05), and that in intraepithelial neoplasia was lower than normal gastric tissues (P<0.05), but there was no significant difference between intestinal metaplasia and intraepithelial neoplasia, or between intraepithelial neoplasia and cancer tissues (P>0.05). The Jun B expression intensity in pateins with tumor infiltrating muscular layer, having lymph node metastasis, or staging in TNM III to IV period, was significant lower than that with tumor limiting in mucosa and submucosa, having no lymph node metastasis, or staging in TNM I to II period respectively (P<0.05). However, the Jun B expression intensity had no significant difference in the different differentiation degree,different cancer region, different tumor sizes, different age, or different sex (P>0.05).â‘¢There was a negatively correlated between the expression of HMGI/Y and Jun-B in gastric carcinoma (P<0.05).Conclusions: HMGI/Y expression upregulated in intraepithelial neoplasia and gastric carcinoma, and its expression intensity was positive correlated with clinical stage, lymph node metastasis and infiltrating depth of gastric carcinoma, while Jun B was opposite. There was negatively correlated between HMGI / Y and Jun B expression. HMGI/Y and Jun B may play an important role in cancerogenesis and development of gastric carcinoma.
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