The Effect Of Low Energy Of Semiconductor Laser Irradiation On Aqueous TGF-beta 2 Expression And It's Damage Study

Objective:To establish the use of 650nm semiconductor laser in the clinical treatment of myopia by the early experimental study of animals.Through different energy parameters of the semiconductor laser in rabbit eyes exposed to different ways to observe whether the 650nm semiconductor laser on the eye can cause the organization of pathological damage,as well as the concentration of TGF-β₂ in the aqueous.Methods:27 rabbits were randomly divided into a normal control group A and four additional groups depending on the power of the laser beam that was used:2 mW beam,group B,5 mW beam,group C,10 mW beam,group D,and 15 mW beam,group E.Except for group A,the others groups were subdivided into team G(acupoints irradiation)and team H(transpupillary direct irration)based on the exposure pathway. Using the low-energy diode laser to radiate on acupoints,such as Jingming, Cuanzhu,Chengqi,Taiyang around the eyes Treatment period was 12 days,once time a day,after 2 days,carried out the second course of treatment.At the beginning and the end of the experiment,recorded the flash electroretinogram(fERG).All the rabbits were monitored by slit-lamp examination and detected the TGF-β₂ concentration of aqueous via ELISA at one day,seven days,fourteen days,twenty-one days and twenty eight days.After the second line treatment,observed the histological changes of retina under light microscope and transmission electron microscope examination .Result:(1) Slit-lampobservations The experimental group and the control group had no lesions of the cornea and lens.(2)Electrophysiological test results Different power semiconductor laser irradiation on the a-wave amplitude changes in the value of statistical significance, in which C group and B group were significantly different (P = 0.015); different power semiconductor laser irradiation on the b-wave amplitude Change the value of statistical significance; different b-wave radiation on the potential value of changes in statistical significance.⑶ELISA test results In different ways of semiconductor laser irradiation on aqueous TGF-β₂ concentration the effect of statistical significance.â‘·Light microscope examination Acupoint irradiation group G is mainly caused by the outer nuclear layer and photoreceptor cell layer of the change in number of photoreceptor cells dense granules, were hill-shaped bulge, making internal and external plexiform layer thinning, are not clear hierarchy. Transpupillary H is the direct exposure group in addition to changes in outer nuclear layer, inner nuclear layer cells also can be seen with disorders, cell particles less, part of neural epithelial cells vacuolated degenerative changes occur.⑸The results of electron microscopy Points inside and outside the control group G closely with nuclear layer cells, in the high-energy group segment happen with minor disorder, minor expansion of the endoplasmic reticulum, mitochondrial swelling. Transpupillary H both inside and outside the direct exposure group showed a small amount of nuclear layer karyopyknosis, loose cells, some ganglion cells rupture of mitochondrial cristae, vacuolization degeneration.Conclusion:The different power of 650nm semiconductor laser irradiation on rabbit visual cells have different response, 2 5mW still in the safe energy range,surpass 5mW will damage the retina.Different way of exposure has different response, compared to transpupillary,acupuncture points have a more significant advantages.In the framework of 2 15mW energy,the concentration of TGF-β₂ can be changed by 650nm semiconductor laser acupoint irradiation.