Experimental Study On Anti-ureaplasma Urealyticum And Bacteriostatic Effect Of Gossypol, Copper-complx-Ion And Their Mixed Liquor

Partâ… Experimental study about inhibitory effects of Gossypol, Copper-complex- Ion and their mixed liquor on international type strain of ureaplasma urealyticumObjective: To study the inhibitory effects of Gossypol, Copper-complax-Ion and their mixed liquor on ureaplasma urealyticum and provide the experimental proof for prevent Uu infection and clinical use of a kind of new functional IUD.Method: The minimum inhibitory concentration(MIC) of Gossypol,Copper-co- mplax-Ion and their mixed liquor was determined on three serotypes of UU with microdilution method.Result: Gossypol, Copper-complax-Ion and their mixed liquor all had significant inhibitory effects on UU(the MIC was 128mg/L, 32mg/L,16mg/L), their mixed liquor especially remarkable on UU3(the MIC was 4mg/L).Conclusion:Gossypol, Copper-complax-Ion and their mixed liquor had significant inhibitory effects on UU, especially their mixed liquor most obvious inhibitory effects on UU3.Partâ…¡Study on bacteriostatic effect of Gossypol, Copper-Complex-Ion and their mixed liquor by different methods in vitro. This part includes two Experiments Experiment One Testing Candida albicans Susceptibility by Gossypol, Copper- Complex-Ion and their mixed liquorObjective: To study the inhibitory effect of Gossypol, Copper-Complex-Ion and their mixed liquor on Candida albicans in vitro.Methods: The minimum inhibitory concentration(MIC) of Gossypol,Copper- complax-Ion and their mixed liquor was determined on Candida albicans with microdilution method and agar dilution method.Result: The minimum inhibitory concentration(MIC) of Copper-complax-Ion was128mg/L.Conclusion: Copper-complax-Ion has a significant inhibitory effect on Candida albicans.Experiment Two Testing E-coli Susceptibility by Gossypol, Coopper-complex-Ion and their mixed liquorObjective: To study the inhibitory effect of Gossypol, Copper-Complex-Ion and their mixed liquor on E-coli in vitro.Methods: The minimum inhibitory concentration(MIC) of Gossypol, Copper- complax-Ion and their mixed liquor was determined on E-coli with Broth macrodilution method and agar dilution methods.Result: The minimum inhibitory concentration(MIC) of Copper-complax-Ion was128mg/L. Gossypol has some inhibitory effect when it's 128mg/L and it's 64mg/L that their mixed liquor has some effect.Conclusion: Copper-complax-Ion has a significant inhibitory effect on E-coli , gossypol and their mixed liquor have some inhibitory effect.