Expressions And Relations Of HMSH2,PTEN And K-ras In Endometrial Cancers

Objective:Endometrial carcinoma (EC)is the most common malignant tumor in females genital system, which threatening the health of women, but its carcinogens are still not clarified, and then effective pathway for predication is deficient. The ealier diagnosis will be increased if the pathogenesy is illuminated. It is usually considered that abnormal ac- cumulation of gene mutation result in tumorigenesis, which includes the deletion of mismatch repair genes(MMR) and the mutation of tumor suppressor genes. There is a complete DNA repair system in normal cells. DNA cannot be repaired exactly and promptly that result in the abnormal accumulation is the result of DNA repair system's functional defect. MMR can repair the mismatched bases and maintain the fidelity during DNA replication, The hMSH2 protien can recognize the mismatchede bases during DNA replication, initiate and participate in the mismatch repair process, and its deficiency may lead to the accumulation of DNA replication error and gene mutation.The occurrence of Ec is related to mutation of many tumor suppressor genes, including PTEN and P53. PTEN can control the apoptosis process and inhibit the growth of cell, while the mutation of PTEN may result in loss of its fuction. So all of them may participate in the occurrence of EC. The experiment is designed to determine the expression of the detecting about hMSH2,PTEN and K-ras sample and discuss the relationship among some clinic pathological factors with these three genes expression.Method:Endometrial carcinoma with clear pathological diagnosis from 54 patients were collected.Immunohistochenistry was used to detect the protien expression of hMSH2,PTEN and K-ras in carcinoma group and normal Endometrial tissue group. SPSS13.0 statistic software was used to analyze the correlation of their abnormal expression to Endometrial carcinoma.Result:In the carcinoma group and normal endometrium group,the po- sitive rate of hMSH2 expression were85.2%(46/54)and 59.1%(13/22),there was significant different between the two groups(p<0.05).In carcinoma, there were not differentiation of the positive rate of hMSH2 between different clinical staging,pathological staging,age,well-moderate differenti- ation and poor differentiation ,infiltrate,and lymph node metastasis(p> 0.05). In the carcinoma group and normal endometrium group,the lack rate of PTEN expression were 63%(34/54)and 27.3%(6/22), there was significant different between the two groups(p<0.05). In carcinoma,the lack rate of PTEN expression in lymph node metastasis and lymph node dismetastasis were58.3%( 28/48) and 83.3%( 5/6) ,there was significant difference between the two group(p<0.05);the expression of PTEN had no relationship with age,infiltrate depth, clinical staging,pathological staging(p>0.05) In the carcinoma group and normal endometrium group,the positive rate of K-ras expression were75.9%(41/54)and 50%(11/22), there was significant different between the two groups(p<0.05). In carcinoma,the positive rate of K-ras expression in different infiltrate depth were69%(29/42)and 83.3%(10/12),there was significant difference between the two group(p<0.05);the expression of K-ras had no relationship with age, lymph node metastasis, clinical staging,pathological staging(p>0.05)Conclusion:1.The expressions of hMSH2 protien was up-regulated in endometrial carcinoma.2.The expressions of PTEN protien was lack in endometrial carcinoma. The expressions of PTEN protien has direct correlation to the lymph node metastasis.3.The expressions of K-ras protien was up-regulated in endometrial carcinoma. The expressions of PTEN protien has direct correlation to the infiltrate depth.4.The expression of hMSH2 and K-ras had closely relationship and they can promomted each other. 5.It may be helpful to detect the protien expression hMSH2, PTEN and K-ras for the predication of endometrial carcinoma.