Expressions Of P53、hMSH2and MGNT Proteins In Endometrial Carcinoma

Endometrial carcinoma[EC] is one of the three common malignant tumor of thefemale reproductive system, its incidence shows a significant upward trend in recentyears, it also shows a trend of young and the disease severely threatens health and life ofthe women, but its pathogenesis is still not clear. The inquiry of its pathogenesis is oneof the important ways to the prevention and treatment of uterine endometrial carcinoma.Gene mutations and injurying often occur in the cell replication process, the cumulationof these mutations can lead to cancer. Endometrial cells is labile cells, which shed andproliferate periodicly, and its gene is periodicly replicated, and the probability of geneinjurying and mismatching in endometrial cell increases greatly, therefore in thereplication process it is repair-dependent. Mutations of p53gene plays a very importantrole in the course of endometrial cancer occurring. Therefore, we hope to make a furtherdisscusion of the mutations in p53gene and their causes to reveal the causes of uterineendometrial carcinoma. Mismatch repair system is one of the currently known five generepair system in the human body, and it is also the more extensively researched one.The hMSH2protein is the key member of mismatch repair proteins, it can identify andrepair the mismatch base. MGMT is able to repair DNA alkylating damage, it is also akind of DNA repair protein.Objective: To detect the expression of p53, hMSH2, MGMT protein inendometrial carcinoma and normal endometrium[NE], we hope to discuss theexpressions and pathological significances of them, analysis the relevance between thetwo repair protein and P53protein，and we hope to make a further explore to thecorrelation of the three index and the clinical significance.Methods: Detected the expressions of hMSH2, MGMT and P53protein of66cases clear pathological diagnosed EC and24NE by immunohisto-chemistry.Analysised the correlation of the three index and the relationship between their expressions and clinical pathology parameters. SPSS17.0statistical software was usedto analyze all the data.Results: The positive expression rate of P53in EC group was43.9%, differedsignificantly with4.17%in NE(P <0.05).The positive expression rates of P53proteininⅠ～Ⅱ pathologic stage EC group were lower than that in Ⅲ pathologic stage, thedifference was statistically significant(P <0.05); the positive rate in I~II clinical stagewas significant higher than that in groupⅢ,there was no significant difference between61.5%in lymphatic metastasis group and39.6%in the group without lymphaticmetastasis(P>0.05); there was a significant difference between34%of shallow musclelayer infiltration group and75%of deep muscle layer infiltration group(P <0.05). Thepositive expression rates of hMSH2in EC were higher than that in NE, the differencewas statistically significant (P <0.05).，and there is no differernce in diffierernt clinicalstage groups, lymphatic metastasis groups、muscle layer infiltration groups and patho-logic stage EC groups. The positive expression rate of MGMT in EC group was71.2%,differed significantly with37.5%in NE (P <0.05). The positive expression in deepmuscle layer infiltration group were significant higher then that in shallow muscle layerinfiltration group (P <0.05). In EC, the expression of hMSH2was positively correlatedwith P53, the expression of MGMT had no correlation with P53.Conclusions:1. The expression rate of P53protein in EC group were significantlyhigher than that in NE group, it may suggest that P53mutation may be one of the causesof endometrial carcinoma. The expression of P53have significant differernces indiffierernt clinical stage groups、muscle layer infiltration groups and pathologic stageEC groups, the results showed that P53protein is an adverse impact factor toendometrial cancer, and it may be related to the prognosis of endometrial cancer.2. The positive expression of hMSH2protein in EC was significantly higher thanthat in NE, suggested that the occurrence of EC may correlated with the mismatch ofgene and its repair failure.3. The positive expression of MGMT protein in EC was significantly higher thanthat in NE, showed that the occurring of gene alkylation damage may be one of thecauses of uterine endometrial carcinoma. MGMT may be related with the malignantdegree of EC.4. In EC, the expression of hMSH2was positively correlated with P53, revealedthat genetic mismatch maybe the cause of mutations in EC.