The Experimental Study Of Suxiao Jiuxin Pellet On Effect The Experimental Of SDF-1/CXCR4 And Anti-Atherosclerosis

Objective:Through establish model of rat with atherosclerosis(AS),This experiment designed to observe the effect of Suxiao Jiuxin pellet(SX) on pathology of morphological changes in AS rat aortic wall and the expression of stromal cell-derived factor(SDF-1),its receptor CXCR4(CXC chemokiner re ceptor-4) on aortic wall,and to further explore it's possible mechanism of anti-AS.Method:Experimental atherosclerosis in rats was produced by feeding with atherogenic-diet and vitaminD3 administration(600,000 IU/Kg by intraperitoneal inject at one time) for 12 weeks.60 male mature Sprague-Dawlays rats were devided into 6 groups randomly and equally:①normal group(N):normal diet;②AS model group:atherogenic-diet;③low dosage SX group(SXL):aterogenic-diet and SX60 mg.kg^-1.d^-1;④mediume dosage SX group(SXM):aterogenic-diet and SX600 mg.kg^-1.d^-1;⑤high dosage SX group(SXM):aterogenic-diet and SX1800 mg'kg^-1.d^-1;⑥Astorvastain group(ATO):aterogenic-diet and ATO4 mg'kg^-1.d^-1.In the beginning, all groups were adaptive fed by normal diet for one week.Then SX or Astorvastain was given by gavaging from the beginning of establish AS model to the end of the experiment.At the same time,normal saline was given to the rats of normal group and model group with the same volume and the same way.After 12 weeks,all rats were killed by breaking away from the spinal cord.Aortas were taken and sectioned quickly.Hematoxylin and eosin(H&E),masson staining were used for morphological investigation by light microscope.Measured intima thickness(IMT),media thickness (MT) of artery stained by HE and artery collagen area(CA),vascular cross-sectional area(CVA) as well as the ratio(CA/CVA) of artery stained by masson respectively with Pro Plus 6.0 image analysis system.The expression of SDF-1 and CXCR4 were examined by immunohistochemistry and western blot.Results:1.Pathomorphological changes in aorta(1) Pathological staining:HE stain:In normal group,the structure of the aortic wall was well-constructed.The intima was thin and endothelia were intact.The smooth muscle cells didn't proliferate.In the model group,Some endothelial cells were lost and the intima thicked.The smooth muscle cells proliferated and lined up in disorder. The structures and arrangements of elastic fibers were in disorder and the fibrous tissues proliferated.Compared with the model group,the pathological changes lessened in the SXM,SXH and ATO groups.Masson stain:In the normal group,the structures and arrangements of elastic fibers were intact and in order.In the model group,the vascular smooth cells and collagen fibers proliferated obviously in the SXM,SXH and ATO groups.(2) Image Analysis Results:The numerical value of intima-media thickness(IMT), media thickness(MT),collagen area(CA),vascular cross-sectional area(CVA) as well as the ratio(CA / CVA) were higher than the normal group,the differences were statistically significant(IMT,MT,CA,CA / CVA:P <0.01;CVA:P <0.05); Compared with the model group,the level of all indictors did not change appreciably in the SXL group(P> 0.05),while the level of IMT,MT and collagen fiber content were declined in the SXM,SXH and ATO group,especially the SXH group and the ATO group(P <0.05),but there were no significant difference between the two groups(P> 0.05).2.Immunohistochemistry Results:The expression of SDF-1 and CXCR4 in the model group were increased when compared with the normal group(P <0.05);Compared with the model group,all treatment group could low-down the expression of the two indictors(all P <0.05),while there was no statistical significance between the SXH and ATO groups(P> 0.05).3.Western Blot Results:The results are basically the same with immunohistochemistry.The expression of SDF-1 and CXCR4 in the model group were increased when compared with the normal group(P<0.05);Compared with M, the expression of SDF-1 and CXCR4 were not influenced in the SXL group(P <0.05), while,both were increased in the SXH and ATO groups(P <0.05).Conclusions:The rat model of atherosclerosis can be established successfully by atherogenic-diet and vitamin D3 administration(600,000 IU/kg by intraperitoneal injected at one time).The result suggest that Suxiao Jiuixn pellet can ameliorate AS pathological changes of rats' artory by resisting and inhibiting the proliferation of AS rats' endarterium,membrane,and collagen fibers,thus delay the process of being AS. The possible mechanism is Suxiao Jiuxin Pellet can inhibit chemokine SDF-1 / CXCR4 axis function.