The Experimental Study Of Scalp Penetration Acupuncture On Effects Of C-Jun After Intracerebral Hemorrhage In Rats

Objective:To observe the influence on the histomorphology change about the big rat brain tissue in acute cerebral hemorrhage by scalp penetration acupuncturing cave and to detect the expression of the c-jun proteinum in the big rat brain tissue in acute cerebral hemorrhage with the methods of immunity histochemistry.To reveal the mechanism about the function of nerve protection by scalp penetration acupuncturing cave.Methods:Selceted the healthy Wistar male rats of 60 by examination. The rats were assigned into 3 sets randomly'model set,pinprick set, inhibitor set,containing 18 rats in each.Each set were again divided into three time phase point of 6 hours,2day,7days,containing 6 rats in each.All rats were made to be the intercerebral hemorrage model.Another 6 rats were assigned as the matched blan kset.The pinprick set were acupunctured on the "Baihui" penetrating "Qubin".We observated the damage extent of the nerve function of each set in deferent time phase point with the neuroethology index;Observated the change of the brain histomophology of each set in deferent time phase point with light microscope;Observated the proteinum expression of c-jun each set in deferent time phase point with the methods of immunity histochemistry.Statistical treatment was made.Results:The detecting result of c-jun with the methods of immunity histochemistry:in the model set,inhibitor set and the pinprick set,the expression of c-jun of rat's brain tissue surrounding the hematoma was increased gradually and to the peak in 2days,but decreased gradually in 7days.In 6 hours,compare with the model set,the pinprick set and the inhibitor set's expression of c-jun havd not the statistical significant (P＞0.05).In the same time phase point of 2days,7days,compare with the model set,the pinprick set's expression of c-jun was lower obviously.Both were relatively showed the significant differene- ce(P＜0.05) and havd the statistical significant.In the same time phase point,compare with the model set,the inhibitor set's expression of c-jun was lower obviously.Both were relatively showed the significant difference(P＜0.01) and havd the statistical significant.It was discovered that the model set's brain tissue surrounding the hematoma was necrosis,dropsy,karyopyknosis of never cells,vacuolization and phlegmasia cells infiltrated with the method of observation under light microscope.The 2days set was most serious.Compare with the model set,the pinprick set and the inhibitor set's damage was abatement in deferent time phase point.Conclusions:Scalp penetration acupuncture cave on the rat of the experiment in cerebral hemorrhage can inhibit the expression of c-jun proteinum of the big rat's brain tissue surrounding the hematoma and can inhibit apoptosis by blocking the signal transduction access of the c-Jun N-terminal kinase,so to achieve the function of nerve protection.