Alfalfa Hypolipidemic Capsule Anti-atherosclerosis Experimental Study

Hyperlipidemia(hyperlipoidemia) that the human body lipid metabolism disorders,plasma lipid concentrations exceed the normal scope of the disease.It is the result of human cardiovascular and cerebrovascular diseases the culprits,the high incidence of human health hazards become the number one killer.As the body abnormal lipid metabolism,increased blood viscosity,and viscous will gradually damage the blood vessels,arteries harden and narrow,causing the final body of the organizations bad blood, which triggered atherosclerosis and other heart and brain Vascular diseases。Atherosclerosis(Atherosclerosis,AS) is a large, medium-sized artery intima there with cholesterol,fat and other types of yellow materials,and more from fat metabolic disorders, neurological disorders and vascular function.As China's people's the level of lives raising and eating habits changing,the disease has become China's leading causes of death.Its main pathological changes in the circulatory system is a large elastic arteries(such as the aorta) and medium-sized elastic muscle arteries(coronary and cerebral artery suffering from the most),suffering from endometriosis artery disease,having a number of diseases existed, Including local lipid and complex carbohydrates are accumulation, bleeding and thrombosis,fibrous tissue become proliferation and calcinosis,and the middle artery calcification and the gradual degradation.Modern cellular and molecular biology techniques, diseases have a smooth muscle cell proliferation,a large number of collagen fibers,elastic fiber and protein polysaccharides,and other connective tissue matrix,the internal and external of the cell lipid accumulation.Therefore,high blood lipid control for the treatment of atherosclerosis is a very important way.This researcher is that making the domestic different AS animal models with the Chinese medicine theory,using of modern medical technology and experimental methods,from the overall model from the animal,the aorta,heart,liver,kidney and other different levels of the system Researchers to demonstrat the effect of capsule of alfalfa in hypolipidemic.In accordance with the "Chinese medicine,natural medicine, acute toxicity of technical guidelines"(2005 version),fromweight, food intake,the appearance of observation and other organs with the eyes to execute directly,the study of alfalfa acute toxicity; accordance with the "Chinese medicine,Natural medicine,long-term toxicity of technical guidelines"(2005 version),with the modern medical technolage,from blood testing,blood chemistry test,the major organs',such as pathology biopsy and other different aspects of experimental system,found its long-term toxicity.Through these experiments,the capsule afford alfalfa hypolipidemic pre-clinical safety,to provide clinical trials basis.1 alfalfa hypolipidemic capsule Pharmacodynamic StudyUsing balloon injury surgery and giving high-fat diet to make atherosclerosis modle at the same time giving drug for dilivery 90d and high blood lipid model.The atherosclerosis and high blood lipid model of rabbit is given high-fat diet for four weeks and grug for another four weeks.The atherosclerosis and high blood lipid model of golden hamster is given high-fat diet for twelve weeks and grug for another eight weeks.The results showed that high,medium-dose group can be significantly decreased hyperlipidemia rat,rabbit serum TCH,TG, LDL-C levels,increased HDL-C level and significantly decreased atherosclerosis index(AI),and with significant difference to Model group.Rabbit high-dose group was significantly lower serum ET,and higher serum 6-keto-PGF1a level.Rabbit high,medium-dose group were significantly lower in the liver organ coefficient. Oberserve aortic from eye:the control group of aortic lumen is smooth integrity.In the model group aortic intima are from mung bean size of the needle to the radio or irregular yellow patch or lipid stripes,and lesions are mainly located in the artery exports and blood vessels branch bending in the convex,clearly visible uplift endometrial surface gray patch,local thrombosis.The treatment group compared with the model group,the pathological changes significantly lighter than the former.Aortic plaque lesions classification,high,medium-dose group was significantly lower area of atherosclerosis to compare with the model group there were significant differences,and to the gypenosides glycosides film the similar intensity.Coronary artery disease classification, treatment group can significantly reduce the incidence of AS and plaque on the degree of obstruction of the lumen,compared with the model group there were significant differences,and the similar intensity of gypenosides glycosides film.Aortic observation:the control group lumen are round,endometrial,and the outer membrane of the film with clear boundaries.The lumen of endothelial cells are single coverage,integrity,and in the film seeing the main spindle smooth muscle cells.Thin outer membrane is the loose connective tissue.Obviously model wall thicken and smooth muscle hyperplasia,the cells disordered,fibrous hyperplasia with patchy or calcification points,elastic fiber layers' structure unclear, a fiber proliferation of atherosclerosis are constructed.The endothelial cells of injury group are falling,thickening and the surface fibers of plaque cap are thin.Prominent plaque are containing necrosis materials,reducing smooth muscle cells, fibrous tissue proliferation with patchy or calcification points.The film are significantly shrinking,which are more typical of mature atherosclerosis.As the dose of medication group increased,endometrial became even more complete,and submerged under a single endothelial cells significantly less than the model group.The smooth muscle layer of film are tidy.Kidney observation:Qu Yuan that the small cells within the lipid reserves so that the cells increased,the lumen stenosis. Mesenchymal also visible in the gathering of scattered foam cells. Liver observation:the naked eye examination found that the normal control group liver were red,sharp edges,flexible.Model group' s liver volume increased and the color was yellow greasy,which were the typical patterns of fatty liver.The drug groups' appearance of the liver treated with the model was no significant difference.Light microscope examination found that the control group was not unusual.Other groups existed in the cytoplasm ranging from the number and sizes of transparent vacuoles.HE staining confirmed the vacuole were neutral fat droplets.Model rats liver showed that diffuse liver steatosis,liver cells changed significantly,a large number of fat cells drops.The drug groups, the liver steatosis lighter in varying degrees than model group, which high-dose group were the lightest of the disease.Heart observation:①subendocardial myocardial diffuse fibrosis.②local myocardial necrosis fibrosis.③local macrophages were proliferation and infiltration of chronic inflammatory cells.The drug groups could significantly reduced the endometrium and in film thickness,compared with the model group there were significant differences.High,medium-dose group and the gypenosides glycosides film had the similar role.These results suggest that alfalfa hypolipidemic capsule is safe, low toxicity.By regulating blood lipids and reducing AI,serum ET,6-keto-PGF1a and liver organ coefficient,AS incidence of lesions,reducing the aorta,heart,liver,kidney pathological changes to treatof atherosclerosis.