Quality Standard And Chemical Composition For Trillium Tschonoskii Maxim

Trillium tschonoskii Maxim.is a medical plant from Trillium genus,Liliaceae,use it's dry root and mature fruit as the medicinal parts.The plant is belonging to the three-level of national plants.Mainly distributed in shenongjia,in Hubei province;enshi autonomous prefecture; yunyang;middle and south of Sichuan province;southeast of Gansu province;north of Yunnan province.As one of the Chinese herbal medicine Trillium tschonoskii Maxim.has the effectiveness of calm and soothe the nerves,promoting blood circulation to stop bleeding and antidote.Trillium tschonoskii Maxim.has lots of steroidal saponins and amino acid,Modern pharmacological research shows that the steroidal saponins is not only can hypolipidemic,anti-tumor hemostasis,immunomodulator,but also use as the main raw materials which extracted in drug intermediate.The research of this article take diosgenin as breakthrough,first of all,study on the extraction, choose the best extraction of Trillium tschonoskii Maxim. by orthogonal design.The best extraction is that:using 70%alcohol and refluxing and extracting in boiling water with 2 hours.And then to study part of the medicine quality standards,which including the medicinal traits,water determination,ash determination,water-soluble extract, alcohol-soluble extract and determination.This article use diosgenin as reference substance,to develop a HPLC method for the dermination of diosgenin in Trillium tschonoskii Maxim..Samples were achieved and separated by Agilent HC-C₁₈(4.6×250mm;5μm) with methanol-water(95:5) as mobile phase.The UV detector was set at 209nm.The column temperature was ambient temperature.The result is that diosgenin was well separated.The present paper describes the isolation,structu ral elucidation and identification.The chemical const ituents were isolated and purified by solvent extracti on together with various chromatographic techniques.W e got ethyl acetate fraction,and then according chrom atogram isolution(silica gel and sephadex LH-20),we got two compounds.On the base of chemical evidence an d spectral data(IR,¹H-NMR,¹³C-NMR,MS),two compounds were identificated as 3-0-α-L-rhc-mmopyranosy(1→3) s tigmasteroside and 3-0-β-D-glucopyranosy(1→3)-α-L- rhc-mmopyranosy-diosgenin.According to the results,Trillium tschonoskii Maxim.is always very broad market prospects of plant.In this paper,preliminary study laida foundation for further research which direct in Germplasm resources of medicinal materials research.And this study provides resources of the development and utilization of herbal medicine.