The Establishment About Nerve Injury Influence On Long Bone Fracture Of The SD Rat Model

Bone fracture heals to be subjected to various factor influence, among them, the nerve factor heal to have an important function in the process in bone fracture, they can regulate various reactions in bone fracture repair process and influence cell to propagate, migration and release.Usually meet in the clinical work the companion has nerve harm(include central nervous system harm and the peripheral nerve harm) of four limbs bone fracture, its bone fracture heals time is more obviously short than pure four limbs bone fracture, and carry in bone fracture usually can observe to have a great deal of osteotylus excesssive growth, even appear a different bone to turn in the muscle, but the osteotylus of the bone fracture part of this kind of sufferer characteristics, is just that the formation speed of the osteotylus is quick, the quantity of osteotylus is many, but the quality of osteotylus cannot compare with non- nerve harm of four limbs bone fracturer.Pass the animal model that this experiment establishment SD big rat grows tube-like in shape bone fracture merger nerve harm(central nervous system+outside peripheral nerve), and build up the pure SD big rat to grow tube the form bone fracture animal model, and the treatment research of merging four limbs bone fracture to provide an experiment data for aftertime's therapy and research.Objective:Research SD rats femoral fracture of the model is established combined nerve injury.Methods:Choose the 32 Marches SD big ,the female and male is unlimited and random divide equally for four sets of, A set:Pure shaft of femur fracture;B set: Shaft of femur fracture merges central nervous system harm; C set: Shaft of femur fracture merges the surroundings nerve harm set;(sciatic nerve, the nerve hurt) D set: Shaft of femur fracture merges nerve harm set;(central nervous system+surroundings nerve)Use bone fracture to build a mold support to result in framework bone fracture, the spinal cord harm makes use of reformative of Alien equip making the T10 spinal cord acute bruise animal model, understand a bone fracture type and move a healing of degree and bone fracture circumstance through a X line, histology check after building a mold.Results:The total SD big rat is 32, the postoperation die 4, the livability is 87.5%. Among them, the central nervous system set die 2, the surroundings nerve set dies 1, pure shaft of femur fracture set dies 1, the X claps a slice of confirmation bone fracture as the crossing or the short inclined type after the postoperation, bone fracture for typical model of two expect to heal process.ConclusionUsing a reformed simplified fracture apparatus and modified Allen's method making the T10 spinal cord acute injury,is a high success rate,is stability and repeatability, and the soft tissue hurts little.