The Establishment Of Hemorrhagic Shock-Microcirculation Observation Platform And Its Application In The Blood Substitutes

Hemorrhagic shock is a common acute and severe clinical disease, while the blood flow of microcirculation over the body is sharply reduced, cells are generally hypoxic damaged and vital organs are characterized by a pathological process. It is the most common cause of early trauma death. The treatment of hemorrhagic shock is usually to transfuse blood or such solutions that can replace the function of the blood to the body. And the curling results are directly related to the using of monitoring tools and monitoring indicators. This article focused on the establishment of a platform which can conveniently and accurately monitor the state of hemorrhagic shock and its application in the blood substitutes researching.With regard to this study:Objective: Establishing a platform to observe hemorrhagic shock - microcirculation model and studying its application in the blood substitutes.Methods: Firstly, we use the Wiggers' Improvement Act, and exsanguinate to make the mean arterial pressure low to 35±5mmHg through the femoral artery in rats for 60 min, then a hemorrhagic shock rat model was duplicated. Secondly, making a microcirculation observation window model on the back of rat, and then combining the two together to create a joint platform. After infusion resuscitation fluid through the femoral vein to the body, we can monitor the continuous real-time changes of the blood pressure and heart rate by the multi-channel physiological that were connected to the other side of the femoral artery cannulation, meanwhile you can observe the distribution of microvascular blood flow and blood flow changes by a microscope.Results: The effective rat model of hemorrhagic shock and microcirculation observation model was duplicated, and achieved a good combination of both. When infusing resuscitation fluid to rats, we observed the restoration of blood pressure and heart rate. And through the micro-circulation model, we observed that microvascular blood flow reduced during shock, the flow rate slowed down, some microvascular were closed, and blood vessels were re-expansion ,blood flow restore after resuscitation .Conclusion: The application of hemorrhagic shock - microcirculation observation platform could be better monitoring the change of the body in the process of hemorrhagic shock and can be easy to take remedial measures to get a better therapeutic effect finally, but also providing a good platform to researching blood substitutes.