Intravital Observation Model Of Dorsal Microcirculatory In Rats And Its Application In Hemorrhagic Shock Research

The microcirculation observation plays an important role in basic physiological research and clinical diagnosis.The intravital observation of microcirculation means directly observe the shape of the microvessel, the expand and contract movement, the flowing speed of the blood, the course of flow and exudation of the red cells and white cells through a microscope. Through the intravital observation of the change of organ microcirculation, we can directly know the pathologic change and the pathogenesis of the disease and then find a method to cure the disease and guide the clinical work. All these deserve extensive research which are prosperous. The intravital observation model of dorsal microcirculatory chamber (DMC) is referred in such basic research and clinical diagnosis as the concrescence and repair of the organ injuries, the net structure of the vessel, contagious diseases, dermatoses and haematology.At present, we mainly study on blood substitutes.On the study of blood substitutes , an important content of the pharmacodynamic evaluation of the blood substitutes is that whether it can effectively resuscitate the serious hemorrhagic shock. And after the resuscitation, the change of microcirculation is one of the important physiological index. In our study, the created model of dorsal microcirculatory are applied in the observation of the anabiosis of hemorrhagic shock and satisfactory observing results are gained, which provides a good basis for future research.About our study:Objective:To establish intravital Observation model of dorsal microcirculatory and apply it in the hemorrhagic shock research; observe the change of microcirculatory physiological index under different physiological states.Methods : SD rats less than 250g, we establish the model of dorsal microcirculatory by making use of dorsal microcirculatory chamber (DMC) and observe the change of such index as the shape of capillary,microvessel branchs,microvessel diameter,bloodstream state and bloodstream color under three different groups of resuscitation fluid (control, hemorrhagic shock, in three different groups of Saline and autologous whole blood and artificial RBC).Results:1. According to statistics, p<0.05, 1) under the condition that there is no distinct differences between the original MAP and the weight of the rat, microvessel diameter are significant deviation in the state of control and hemorrhagic shock. 2) After resuscitation treatment,statistic analysis indicated that there is no significant deviation between autologous whole blood and artificial RBC; while the rest two groups are quite different from cotrol and there are distinct differences among each group. 3) After resuscitation treatment, the experimental groups are significant deviation from the control in microvessel branchs and there are differences among each group. 2. In the course of hemorrhagy, as time goes by, when MAP keeps about 110mmHg,80mmHg,60mmHg,45mmHg,35mmHg, we can clearly see bloodstream state at seven grades and the distinct change of the shape of the capillary.3. On observation, there are distinct differences in bloodstream color between the state of control and hemorrhagic shock. When we input resuscitation fluid, it reverts to the state before shock but not exactly the same state.Conclusion:The intravital observation model of dorsal microcirculatory can directly reflect the real-time change of the physiological index of microcirculation in the couese of experiment. The observing and statistical methods are simple. And when applied in hemorrhagic shock, it can effectively evaluate each different kind of resuscitation fluid.