The Study About The Role Of Dendritic Cell In Allergic Rhinitis Mice

ObjectiveTo study the role of dendritic cell (DC) in allergic rhinitis and the relationship between allergic rhinitis and allergic asthma.MethodsBALB/C mice, 6～8 weeks old, were randomly divided into two groups: allergic rhinitis (AR) group and control group. AR model in mice were established by ovalbumin(OVA) intraperitoneal immunization and nasal antigen challenge. Behavior score and the routinely HE staining of the nasal mucosa were used to evaluate the AR model. DC numbers were evaluated by immunohistochemistry. The mice in the AR group and the control group, received an intranasal injection of immature DC obtained from the bone marrow and marked with Hoechst33342. Then we traced the immature DC by immunofluorescence histochemistry to observe the changes of the injected DC. ResultsThe behaviors of the AR mice were different from the mice of control group. Increased numbers of DC can be found in mice with AR. The immature DCs which were injected intranasaly distributed in the lamina propria and epithelium of the mucosa in AR mice and turned to be mature ones . In one of the AR mice the injected DCs transferred to the lung. While in the control group , the injected DCs stayed to be immature ones in the epithelium of the mucosa. No injected DCs were found in the lung of the mice in the control group.ConclusionsDC may play an important role in allergic mice. And the allergic rhinitis maybe induce asthma by the migration of DC.