The Experimental Histopathologic Change Of Applying Suramin To Filtering Surgery In Rabbits

Objective To study the histopathologic changes of filtering blebs after applying suramin after trabeculectomy in rabbit eyes.Method Twenty rabbits were randomly divided into two groups, each group consisting of 10 rabbits. Trabeculectomy with application of suramin was performed on one eye and trabeculectomy alone on the other eye in first group. Whereas, trabeculectomy with application of mitomycine(MMC) was performed on one eye and trabeculectomy with application of suramin on the other eye in second group.Histopathologic changes in filter bleb were investigated by immunohistchemistry, Hematoxylin-Eosin Staining and Picrosirius Red Staining at 3 days,7 days, 15 days,30 days, 60 days after surgery.Results Fibroblasts: The numbers of fibroblasts in eyes after trabeculectomy with suramin as well as trabeculectomy with MMC were significantly reduced (p<0.05) compared to eyes with trabeculectomy alone. There was no significant statistical difference in the numbers of fibroblasts between trabeculectomy with suramin group and trabeculectomy with MMC group. Newly formed collagen fiber: Immunostaining of collagen fibers showed that the numbers of newly formed collagen fibers in trabeculectomy with suramin and trabeculectomy with MMC group significantly decreased compared to trabeculectomy alone group. However, there was no significantly statistical difference between numbers of fibers in trabeculectomy with suramin group and trabeculectomy with MMC group. Neovascularization of filtering blebs: immunostaining showed that the numbers of new vessels in trabeculectomy with suramin decreased compared to trabeculectomy with MMC group and trabeculectomy alone group;there was no significantly statistical difference between numbers of neovascular in trabeculectomy alone group and trabeculectomy with MMC group.Conclusion Trabeculectomy with applying suramin may enhance the success rates of surgery by suppressing the fibroblasts and synthesis of newly formed collagen fibers,and by inhibiting scar formation.The effect of Suramin was approximative to MMC...