The Correlation Between Gentamicin Usage And Higher Morbidity Of Asthma And It's Mechanism

Objective: To study the correlation between use of gentamicin and morbidity of asthma, and explore the mechanism of which.Methods: 32 neogenetic SD rats were randomly assigned to 4 groups: group1 (control group),group2 (gentamicin group),group3 (asthma group) and group4(gentamicin and asthma group). Rats in group 2 and 4 received gentamicin by lavaging twice per day, started in the first 24 hours after birth, and continued for two weeks. Rats in group 1 and 3 received identical physiologic saline instead. Six weeks later, Rats in group 3 and 4 were sensitized by subcutaneous injection of OVA and algeldrate and peritoneal injection of bacillus pertussis, and by atomization of OVA. Rats in group 1 and 2 received identical physiologic saline instead.The following indexes were detected:(1) SD rat ethological signs of asthma: Such as the frequency of sneezing,gripping nose and the extent of gasping.(2) The development condition of lymph node: The rats'mesenteric lymph node pathological slice and HE stain were made.(3) The inflammation in the lungs: The lung pathological slice and HE stain were made.(4) The concentration of IL-17 in the each rat's blood serum: With the method of ELISA.(5) The relationship between concentration of IL-17 in the serum and scores of the inflammation in the lungs: With the statistics method of linear regression and correlation. Result:1 Ethological signs of rats: The rats in group 1 and 2 had no signs of sneezing,gripping nose and gasping. Inversely, the rats in group 3 and 4 had apparent signs of sneezing,gripping nose and gasping(p<0.05). The above signs were more apparent in group 4 than those in group 3(p<0.05).2 The development condition of mesenteric lymph node : The mesenteric lymph node of control group(group 1) had a normal histological characteristic, but in group 2, the normal layer structure including nodule cap, light region and dark region of the lymphatic node disappeared, substituted by simple dark region, and the adjacent lymphatic nodes were prone to confluence, the deputy cortex area expanded and proliferated, in the medulla area, the medulla cord proliferated slightly, and many dense materials deposited in the medulla sinus.3 The inflammation in the lungs: The histological constructions of lungs in group1 were normal. In group2, the slight epithelium injury and alveoli edema could be observed in some rats, but couldn't be observed in other rats (p>0.05) .Contrast to the control group, significant inflammatory infiltration existed in the group 3 and 4 (p<0.05), and the semi-quantitative scores of the inflammation of the lungs in group4 were higher than those in group 3(p<0.05).4 The concentration of IL-17 in the blood serumis: Compared with the concentration of IL-17 of rats in the control group, the concentrations of IL-17 in group 2 increased slightly (p<0.05), and augmented in group 3 and 4. We also found that the concentration of IL-17 of rats in group4 was higher than that in group 3(p<0.05).5 The relationship between concentration of IL-17 in the serum and scores of the inflammation in the lungs: A linearity relationship presented between the concentrations of IL-17 of the blood serum and scores of the inflammation in the lungs. This indicated that the concentration of IL-17 in serum could indirectly reflect the level of inflammation in the lungs.Conclusion:Administration of gentamicin during rat's childhood might do harm to the development of immune organ due to less opportunities of accepting immune stimulation, thereby, increased the risk and morbidity of asthma. This study might exploit one new field on anaphylactic diseases such as asthma.