| Objective:Renal cell carcinoma(RCC) is contemporarily the common malignant tumor in urology, which originates in renal tubular epithelial cells. RCC is the most common malignant renal parenchyma,of which about 70% ~ 80% are renal clear cell carcinoma(RCCC). Its generation and development is regulated by a variety of genes. Therefore, it is extremely important to do the researches of the genes on pathogenesis ,diagnostic criteria and the treatment, which are related to RCC. The pim-1 encoding a serine–threonine kinase, has been found to be related to tumor generation and development. The protein c-Myc encoded by the c-myc proto-oncogene is a important transcription factor of eukaryotic cell, which has dual effects in both cell proliferation and induction of apoptotic. We can find that the expression of c-myc can be changed in many tumors, such as leukemia, lymphoma, colon carcinoma, mammary cancer. Pim-1 and c-myc play important roles in apoptosis, proliferation, differentiation and tumor generation. The research also shows that there is a synergistic effect of the expression in T, B lymphoma and prostate cancer between pim-1 and c-myc , but it has not been reported that two proteins are expressed in RCC simultaneously and relationally. By detecting expression of pim-1 and c-myc in different clinical stage and pathologieal grade of RCC, investigating the relationship between the expression and cancer tissue invasion, metastasis and prognosis, and also investigating the correlation between pim-1 and c-myc. It can provide new clues and ideas for studying the biological behavior of RCC ,and provide theoretical basis for exploring new drugs and methods of curing RCC as well.Materials and Methods:1 Materials:The study selected 80 cases of RCCC which from 2004 to 2008 in Bethune international peace hospital. There were 56 male and 24 female,with an average age of 55.9 years(rang 18 to 82 years). The subjects were classified into four grades according to the Fuhrman pathological criteria,in which,12cases were Gl,21cases were G2,38cases were G3,9cases were G4. According to AJCC phases criteria,11cases were phase l,24cases were phase ll,36 ases were phase lll,9 cases were phase VI. In addition, 20 cases of normal renal tissues beside the renal carcinoma and 8 cases of normal renal tissues (which were cut from the kidney suffer the other diseases) were take as reference.2 Methods:We studied the expression of pim-1 and c-myc from the specimens we selected by PV6002 immunohistochemical technique.The reagent we used were mouse monoclonal antibody pim-1 and c-myc. Specimens were colored by DAB and redyed by hematoxylin. We selected four vision randomly and every vision contained 100 cells . The score we calculated were maked with A and B according to staining intensity and extent , the final score was AxB. The score≤1 was negative expression, and the score>1 was positive expression.3 Statistical analysis:All the data were analyzed with SPSS for Windows 13.0 software.The comparison between group positive rates was examined by X2 test(p<0.05).The correlation between pim-1 and c-myc was analyzed by Spearman rank correlation analysis.Results:1 The pim-1 stain located on cytoplasm,rarely on nucleus and membrane. In 80 pieces of RCCC tissue,the positive expression of pim-1 was 69(86.7%); in 8 normal renal tissues and 20 cancer adjacent tissues , the positive rates was 25% and 35% respectively. There was significant difference about the expression of pim-1between RCCC tissue and other groups , but not in normal renal tissue and cancer adjacent tissue.2 The c-myc stain located mostly on cytoplasm,partly on nucleus. In 80 pieces of RCCC tissue,the positive expression of c-myc was 68(85%); in 8 normal renal tissues and 20 cancer adjacent tissues , the positive rates was 25% and 30% respectively. There was significant difference about the expression of c-myc between RCCC tissue and other groups , but not in normal renal tissue and cancer adjacent tissue. 3 The expression of pim-1 and c-myc had no significant correlation with the age and gender of patient's (P>0.05). But related to the clinical stage and pathological grade. The expression of pim-1 and c-myc in RCC was consistent with each other: the expression of pim-1 and c-myc in G3~G4 grade was significant higher than G1~G2 grade (P<0.05),in 111~IV stage was significant higher than l~11stage (P<0.05),and also higher in the group of lymph node metastasis than those in the group without 1ymph node metastasis (P<0.05). According to the statistical analysis, there was a positive correlation of the expression in RCC between pim-1 and c-myc. (r=0.727,P<0.01).Conclusions:1 The expression of pim-1 and c-myc in RCC were significantly different to in normal renal tissue and cancer adjacent tissue. It showed that pim-1 and c-myc may be closely related to the pathogenesis of RCC.2 There were significant diffrences of pim-1 and c-myc expression in different pathological grades, clinical phases and 1ymph node metastasis(P<0.05). It suggested that the proto-oncogene pim-1 and c-myc may play important roles in the pathogenesis and development of RCC. The detection of their protein could be an objective index for judging the invasive and metastatic ability of RCC.3 There was a positive correlation of the expression in RCC between pim-1 and c-myc . Pim-1 and c-myc may have a synergistic effect in RCC pathogenesis and development.4 Abnormal expression of pim-1 and c-myc may offer the theory evidence for the diagnosis,treatment and prognosis of RCC.
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