Investigating HCV-Ab And Genotypes Of HCV Distribution Among Residents In A "Blood Donation" Village In Hebei Province

Objective: To investigate the prevalence , risk factors and genotype of HCV infection among residents in a blood donation village form Hebei province and to put forward a basic data for reducing transmission of HCV after blood donation and management ,treatment of HCV patients.Methods: A cross-sectional survey of random sample of 520 residents of blood donation village in rural Chenglang village Dazhang countryside Zhao county, Hebei Province, was conducted in 2008. Questionnaires were administered, and 520 blood samples were tested for hepatitics C virus(HCV)antibodies by ELISA, and 5'UTR, C/E1, NS5B of HCV genome of the 483 blood samples were obtained by RT-PCR amplifying, and the gene sequences were analyzed and the polygenetic tree were drawn by the software Mega 4.0.Results: HCV antibody seroprevalence rates were 28.46%(148/520)in all subjects, 42.80%(101/236)in former commercial plasma/blood donors, commercial plasma/blood donors' HCV-Ab seroprevalence was higher than no blood-donation villages(p<0.0001). HCV antibody seroprevalence rates was 15.38% in while-blood donors, 80.00% in plasma donors, 69.30% in both while-blood and plasma donors. Selling plasma was the strongest independent predictor of HCV sreopositivity (P=0.0037 ), but selling while-blood was not(P=0.7736), A history of operation was also independently associated with HCV antibody seroprevalence(P=0.0270).Between whole blood-donation years, times with HCV-Ab seroprevalence there are dose-response relationship, and there are also dose-response relationship between plasma-donation times with HCV-Ab seroprevalence. But, only mixing-donation and plasma-donation times could increase HCV-Ab seroprevalence by multi-factor Logistic analysis. HCV-RNA nucleinic acid Positive objectives were 79, genotyping for 71 samples successfully, including 1b and 2a sub-genotype only, it's number is 37,34 respectively, their rate are 52.11%,47.89% respectively, but remaining 8 samples not being genotyped successfully.Conclusion: unsafe practices during illegal plasma donation led to a high risk of HCV seropositivity for donors during the later 1980s and former 1990s. HCV genotypes among the village residents were 1b and 2a two subgenotypes only.