| AIM:Alzheimer's disease(Alzheimer,s disease,AD) is a kind of happened during the early old age or old age of a chronic progressive neurodegenerative disease.It is a common,frequently-occurring disease.The pathological changes are mainly the cerebral cortex, hippocampus,basal ganglia,such as atrophy,a variety of neurons, especially cholinergic neurons of the primary variability,from the formation of amyloid deposition in senile plaques and neurofibrillary tangles,the emergence and an increase.Its primary clinical symptoms are intelligence,memory,sensory-oriented capabilities,sense,language,thinking ability of irreversible degradation carried out,and there is often accompanied by personality changes,and its incidence increases with age.At present,for the clinical treatment of AD there is no specific drug therapy is generally used to improve cognitive function and brain metabolism of some to promote the drug to control symptoms,but it does not slow down or to save the large variety of neurons, especially cholinergic neurons The primary element degeneration. So,look for a safe,innovative,convenient and economical treatment method has become the urgent need for the treatment of AD.In recent years,acupuncture AD due to better improve the cognitive function status,and non-toxic side effects,improve the overall quality of life in patients with certain advantages,and has as a unique,safe,convenient way to have more and more of the treatment of many Clinical applied to obtain a more positive effect. To study this subject for electricity AD hippocampus of rat brain glial cell line-derived neurotrophic factor and GFR-αl receptor expression to explore the role of electricity for the treatment of AD and its role in the mechanism,in order to provide experimental basis for clinical acupuncture.Method:With 10%chloral hydrate(4ml/Kg) by intraperitoneal injection of anesthetized rats,Cut off the head skin,flat head bit fixed WDT- H in rat microelectrode brain boosters on three-dimensional positioning to dynamic operation iodine skin disinfection,along the sagittal line of the middle cranial cut scalp and periosteum,and then hydrogen peroxide in a small cotton ball lightly scripe noodle bone applying fully exposed before the skull.Reference to Paxinos,such as bilateral hippocampal map to determine coordinates of two injections:the first point for the former before the skull 3.5 mm,the center line the right side of 2.0mm,subdural 2.7mm;the second point for the former before the skull 3.5mm,the center line of left lateral 2.0mm,subdural 2.7mm. With dental drill according to the above two coordinate drilling (diameter about 1mm),then to micro-syringe for each injection point Aβ25-35 3μl.Specific methods:micro-5μl syringe to extract 3μl Aβ25-35 solution(5μg/μl,containing 0.2%Vit C), will be fixed in the syringe vertical microelectrode WDT-Ⅱthrusters on the brain three-dimensional positioning,slow needle (injection speed 0.5μl/min),leaving the needle injection end of 5min,in order to slow 1.0mm/min back pin.After two injections skull gelfoam filling holes,stitching the scalp;sham-operated group using the same method of operation and the coordinates of injection with only 0.2%Vit C in normal saline.Nodel around the Norris water maze after the behavioral testing,2min Record in locating the platform in rats,2min to find the platform model is the success of AD.The success of the model rats were randomly divided into model group and EA group.Electro-acupuncture group Wind Point taken for treatment.After treatment by immunohistochemical detection of hippocampus in each group glial cell line-derived neurotrophic factor and 6FR-α1 receptor positive cells.Test results through the SPSS 11.0 statistical package deal.Result:(1) after the AD model rats with an average escape latency than the obvious extension of the previous model;electric needle AD model rats can significantly shorten the incubation period(2) after the AD model rats with the number of cross-platform than a marked increase in the previous model;electric needle AD model rats can have a marked increase in the number of Units;(3) electro-acupuncture group hippocampal glial cell line-derived neurotrophic factor and GFR-α1 positive neurons in quantity than the model group and normal group was significantly increased(P < 0.05).Conclusion:(1) electro-acupuncture Fengfu AD model can enhance the expression of GDNF in hippocampus increased significantly,up to a certain extent in order to prevent the cholinergic neurons in the process of apoptosis,the purpose to protect the brain;(2) electro-acupuncture Fengfu AD model may give rise to hippocampal expression of GFR-α1 significant increase in damage may indirectly promote more GDNF cells to uptake and internalization;... |
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