| The effective substances of Chinese multiherb remedy is refers to the total compounds which have effective function to some disease. These effective substances'sources are complex, including coming from raw material of herb medicines, the products in preparation process (for example to processing, decoction process) and so on. The effective substance of Chinese multiherb remedy is not to combine the substances in herb medicines mechanically, but is the result of many kinds of effective substances'interaction.The Chinese multiherb medicine Xiaochaihu-tang is composed of seven herb medicines and was first described in Shang Han Lun by Zhang Zhongjing (150 to 219 A. D. in the Chinese Eastern Han Dynasty). Xiaochaihu-tang (or Shosaiko-to in Japanese) has attracted a great deal of attention for its possible healing effects on chronic hepatitis and its beneficial effect to prevent the development of hepatocellular carcinoma in patients with cirrhosis of the liver. In addition, many studies in animal models or on cell lines have demonstrated that Xiaochaihu-tang or its ingredients possess cytoprotective effects on experimental liver injuries, an antiinflammatory effect on chronic hepatitis, preventive and therapeutic effects on experimental hepatic fibrosis via the inhibition of hepatic stellate cells, and lipid peroxidation in hepatocytes.Because of the complexity of Chinese herb medicine, it is necessary to fully understanding the compounds in Chinese herb remedy. A data base of compounds in Xiaochaihu-tang has been established, which including about 500 compounds from seven herb medicines. The exact molecular weight is also displayed. To quickly identify the compounds, a analysis method apply HPLC-TOF-MS has been established and the total analysis time was 42min. In the total ion current chromatogram of Xiaochaihu-tang, 38 compounds was identified and 17 compounds were from Gancao, 10 compounds were from Huangqin, 4 compounds were from Renshen, 4 compounds were from Chaihu, 3 compounds were from Shengjiang, no compound was identified in Banxia and Dazao. As the result, it is conclude that flavone compounds have a large amount in Xiaochaihu-tang and they mainly from Huangqin and Gancao positioned earlily in the chromatogram. Saikosaponin are important compounds in Xiaochaihu-tang, maybe as their small polarity, they appeared late in chromatogram. China pharmacopoeia in 2005 version set baicalin as the qulity control of Xiaochaihu-partical. Because of well ultraviolet absorption, flavone were usually detected with UV detection. Baicalin and wogonoside are two important compounds in Xiaochaihu-tang, their amount will remarkable influence the qulity of it. Reflux extraction was used to produce Xiaochaihu-tang. It is used Agilent Zorbax XDB-C1(8150mm×4.6mm, 5μm)column and ACE/H2O systerm. Compared to China pharmacopoeia, the method is apply to the determination of baicalin and wogonoside meanwhile which will raise the qulity control to Chinese multiherb medicine Xiaochaihu-tang and set foundation to drug metablism and pharmacokinetic research.Most of herb medicine is oral administration by decotion. Actually, the Chinese herb medition will interact during processing and decotong and also in vivo. The method of determination of baicalin and wogonoside in vivo was established with HPLC-MS. The method was apple to the baicalin and wogonoside pharmacokinetic reaserch in rats after oral administration of Xiaochaihu-tang and Huangqin extraction respective. Baicalin and wogonoside both have two absorption peaks. It is supposed that maybe exist enterohepatic cycle. Compared to oral administration Huangqin extraction, the half life of baicalin and wogonoside is more shorter of Xiaochaihu-tang, which suggest the other compounds in Xiaochaihu-tang accelerate the elimination of baicalin and wogonoside.
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