Studing On The Annual Dynamic Of The Microbial Community And The Water Quality Of The Water Cellar In The Karst Mountainous Area Of Southwest China

This research analyzed the variation of water quality in different types of Water Cellars locating in mountain suburb of Southwest area of China. At the same time, this research also analyzed the variation of Microbiology group in the water cellar and the connection between these two variations.The results showed the water quality which kept in hermetic Water Cellar is better than which kept in open Water Cellar. During the long time of keeping water, the water quality which kept in open Water Cellar declined obviously. Mean while, the water quality which kept in hermetic Water Cellar stays steady if supply spring water.This research found the total DNA of microbiology in the water sample directly by the way o(fTENP buffer-PBS-Lysozyme-Repeated freezing and thawing) after collecting colony from water sample percolating with microporous membrane. Moreover the purity of the DNA can be used to do next PCR-DGGE analyses.The results of PCR-DGGE showed that in open water cellar the bio-diversity of colony of microbiology is more obvious than which in hermetic Water Cellar. And between the plentiful water period and the dry period,the changes of colony in open water caller are more obvious than which in hermetic Water Cellar. There are some connections between the variation of water quality and the colony of the microbiology.The result of this paper can help us assess the part placed by microbiology during the changing process of the water quality. These results would provide a scientific basis on deep studying on the relationship between microbial community and the change law of the water quality in the water cellar...