Studies On Quantitative Control Of Fatty Oil Of Raw Materials Of Boxintong Soft Capsule

The original medicines of boxintong come from Allium fisturosum L. var. caespitosum Makio which is a plant belonging to family of lily, genera of shallot. It has been used as folk medicine to treat many diseases.The chemical compositions of raw materials of boxintong (SFE) have been studied by GC-MS method. Eight kinds of constituents were got and identified, which are organic sulfide compounds, organic amine, heterocycles, aldehyde, ketone, ester, fatty acid (ester), and long-chain alkanes, compairing with traditional method steam distillation extraction(SDE). Organic sulfide compounds and fatty acid have a lot of effect on pharmacological aspects and extensive curative effects by researchs and reports.Nine fatty acid compounds were also got and identified by GC-MS. The total content of the fatty acids was 7%. The content of linoleic acid was the highest (62.44%)and palmitic acid was the next (21.34%). In the research of organic sulfide compounds, we analysis their structures by biogenesis pathway.As fatty oil, we determine physicochemical constant of boxintong with method from the Chinese Pharmacopoeia. So that we could provisions the effective ranges of the raw materials: acid value 40~43, saponification value 177~180,hydroxyl value 127~130, iodine value 40~43.A capillary gas chromatographic (GC) method equipped with flame ionization detection was developed for the simultaneous analysis of linoleic acid in boxintong. Compounds were separated on an HP-INNOWAX(30 m×0.53 mm×1 um). The polar column was 120℃for 2min programmed to 180℃at 10℃/min, 180℃for 2 min, programmed to 184℃at 2℃/min, 184℃for 1 min, programmed to 190℃at 1℃/min.;detector temperature 250℃. The orthogonal design was adopted to optimize the derivation reaction conditions of fatty oil. The calibration curves were linear in the range of 0.107~0.321 ug (R2=0.9991) . The average recovery of linoleic acid was 97% ~103% (RSD=1.3%). The assay method was simple, rapid, reliable and reproducible and provided a quantitative basis for the quality assessment of boxintong.Determine the total sulfide element of drugs by elemental Analyzers at the first time. The result shows that its precision was under 3%, and the recoveries ranges were of 99%~104%. The proposed method is rapid and suitable for the determination of total sulfide element. Then all sulfide elements will be converted to organic sulfide 2,5 - dimethyl thiophene the highest content. It is 8.6%.