The Dynamic Change Of Tumor Necrosis Factor-α,Endothelin-1,Nitric Oxide And The Intervention Of Ulinastatin On Rats Which Were Received High Intension Electricity Burn

Objective: To study the pristine dynamic change of tumor necrosis factor-α,Endothelin-1,nitric oxide and the intervention of ulinastatin on rats which were received high intension electricity burn, to provide reference for clinical therapy of high intension electricity burn.Methods:1 Grouping the experimental animals: 144 healthy grownup SD rats, both male and female would work, no pregnant female rats. The high intension electricity burned group (shortened as high intension electricity group): 48 rats; non-high- intension electricity burned group (shortened as referencing group):48 rats; ulinastatin group (shortened as treated group):48 rats.2 Preparation before the experiment: weighting the rats, number them, moulting the rats in inner sides of the left upper limbs, right lower limbs and belly with 8% sulfureted natrium liquor, them use physiological salt water to wash them on dry them, put them in moderate temperature, no food and water for a night3 Copy of high- intension electricity burned models: using 1% amyl barbital natrium liquor to narcosis the rats, after that fix the rats and connect them with circuit. Using TC-30-20KVA booster and YDJ-10KVA experimental transformer, hit them with 10000 voltages, the current would go in from left upper limbs, going by the trunk, and pass out from left lower limbs. Then the entrance and the exit would form. 5 seconds current in, them recover them, after that, the models are done.4 Time to exsanguinate the rats: pre-electroshock(T₀), short after electroshock(<15min, T-1), 1 hour after electroshock(T₂), 2 hours(T₃), 4 hours(T₄), 8 hours(T₅). the treated group need the injection of ulinastatin in belly immediately after electroshock(2WU/Kg), then to exsanguinate them with the method above.5 Checking: put all the blood into centrifuge(3000r/min,10 min), take away the serum, put the serum in frig(-80℃), after the whole work done, check the index. check the consistency of TNF-α,ET-1,NO in plasma on different times respectively, in order to know the change of consistency after electroshock and the effect of UTI. use ELASA to check TNF-α,ET-1, and check NO with the spectrophotometer.6 Experimental DP: statistically analysis all the data with SPSS16.0. all the data should be present in average standard differences, 2 independent samples of different groups should bchecked in t-checking, the even data should be check in its own manner, vice versa. if p<0.05, then it has statistical meaning. Result:1 The content of TNF-α: the content of TNF-αin serum after electroshock in groups of high- intension electricity and treated ones witness an immediate zooming up for 8 hours, but pace of treated group is slower than high intension electricity group; the level of TNF-αin serum of different time, form electroshock to 8 Hours following, the TNF-αlevel is apparently higher than referencing group and non-electroshock ones (P<0.05). Comparison between groups, the treated group is almost the same with other two groups short after the electroshock, no apparent differences statistically (P>0.05), 1 hour later to 8 hours following, the level of TNF-αof treated group is apparently lower than the high- intension electricity group (P<0.05), but still higher than the referencing group.2 The content of ET-1, and the ET-1 's movement tendency. The level of ET-1 in treated group and high- intension electricity group didn't go up both, short after the electroshock. From 1 hour later to 8 hours following, the level of ET-1 in both group witnessed a going up, but the pace of the treated group is slower; the comparison of ET-1 consistency, no apparent differences between 3 groups short after the electroshock. (P>0.05), but from 1 hour to 8 hours following, the level of ET-1 of high intension electricity and treated groups is apparently higher than the referencing group and non-electroshocked ones(P<0.05). Comparison between groups, no apparent differences between treated and high- intension electricity handed groups(P>0.05) short after the electroshock, 1 hour later, the level was lower than the high- intension electricity group (P<0.05), but still higher than the referencing group.3 The content of NO: the comparison of the NO movement tendency, the level of NO in 3 groups are all without apparent changes in different times; the comparison of consistency of NO, the level of NO in 3 groups are all without apparent changes in different times(P>0.05). The comparison between groups, no apparent changes in 3 groups in different times (P>0.05).4 The comparison of ET/NO: ET/NO movement tendency, from short after the electroshock to 8 hours later, the high intension electricity group witnessed an apparent going up, while the treated group witnessed no apparent changes short after the electroshock. from 1 hour later to 8 hours following, the level got higher, but still lower than the high- intension electricity group; the consistency of ET/NO, the high intension electricity group's consistency is higher than the referencing group through out the time (P<0.05), the treated group witnessed no apparent changes when compared with the referencing group short after the electroshock (P>0.05), from 1 hour later to 8 hours following, the level went higher than the referencing group (P<0.05). Comparison between groups, the level of ET/NO of treated group is lower than the high- intension electricity group through out the time (P<0.05)Conclusion:1. The high- intension electricity burned rats exi sted a high level of ET-1,TNF-αin early times, but the NO level is stable;2.the obstacle of the micro circulation of the burn ed rats, probably linked to the high level of ET-1,TNF-αin their serum, not related to the NO level;3.the UTI could restrain the level of ET-1,TNF-αin serum of those burned rats, but has no effect on NO which is under the regular level. the UTI could do better to the microcirculation by lower the level of ET-1,TNF-α. ;4. UTI may have some active clinical meanings in treating the micro circulation obstacle caused by high intension electricity burned cases in their early time.