| Objective Investigate the effect of blood glucose variation on emptying and GHSR mRNA expression in hypothalamus and gastric,then explore the relationship between Ghrelin receptor expression and gastric emptying disorder in type 2 diabetic rats.Methods Thirty Wistar rats were randomly divided ioto three groups:type 2 diabetes group,simple obesity group,normal control group.The type 2 diabetes group was fed by high-glucose,high-fat diet and injecting STZ into peritoneal cavity of rat;the simple obesity group was fed by high-glucose, high-fat diet;the normal control group was fed by usual diat.After STZ injection,the levels of body weighting and blood glucose were detected.Gastric emptying was measured by intragastric administration of phenolred.Moreover,Real-time PCR was used to examine the expression of Ghrelin receptor mRNA in the hypothalamus and gastric.Results At the end of 8 weeks,compared with the controls,body weighting and insulin were increased significantly(P＜0.01) in rats with type 2 diabetes and simple obesity group,blood glucose was increased,but there was no statistical significance(P＞0.05).After STZ injection,insulin was decreased in type 2 diabetes,and there was no statistical significance(P＞0.05) between with the controls,blood glucose was increased significantly(P＜0.01);gastric emptying were delayed in rats with type 2 diabetes and simple obesity group(P＜0.05),the expression of Ghrelin receptor(GHSR) in hypothalamus and gastric of type 2 diabetes were decreased(P＜0.05).Conclusion the model by feeding with high-glucose,high-fat diet and injecting STZ into peritoneal cavity of rat is perfect model of type 2 diabetes.The rats of type 2 diabetes group and simple obesity group have gastric emptying delay and Ghrelin receptor decrease.Moreover there are positive correlation.All of them suggest that the decreased GHSR of hypothalamus and gastric may participate in the delayed gastric emptying.
|
PDF Full Text Request