Polygonum Multiflorum Fingerprint System Components And Characteristics Of The Study Group

In this paper,fingerprint technology of Chinese medicine Polygonum multiflorum Pieces of the HPLC system fingerprinting to compare the different places,different processing methods sliced Polygonum multiflorum fingerprinting system differences in the polarity of different parts of Polygonum multiflorum fingerprint differences;At the same time producing high-pressure effects of different soy and different parts of Polygonum multiflorum Polygonum multiflorum polar model of the role of blood in rats, as well as the serum containing Polygonum multiflorum on granulocyte-macrophage Department of hematopoietic progenitor cells in rat colony cloning, and find the characteristics of component groups,proven system for Limonium multiflorum Thunb role the material basis of the groundwork done.一.Polygonum multiflorum HPLC fingerprint system researchObjective:The establishment of different origin,different processing methods multiflorum Thunb Pieces HPLC system fingerprint,and compare their differences.Method:By Column:AQ-C18 column(4.6mm×250mm,5um) column,linear gradient elution,mobile phase ofacetonitrile-0.01%phosphoric acid solvent system, the detection wavelength was 270nm,column temperature 25℃,Progressive sample 10ul,recording time 70min,samples of each batch chromatogram fingerprint similarity using data analysis software.Result:Pieces multiflorum Thunb system identified a total of eight peaks,set up a total of 16 approved patterns of the samples,a stilbene glucoside content and comparative similarity.On this basis,the research and manufacturing of raw Shouwu Polygonum multiflorum,as well as parts of different polarity difference fingerprint.Conclusions:The use of fingerprint information,can provide a more comprehensive evaluation of the quality system Pieces multiflorum Thunb.Polygonum multiflorum fingerprint processing occurs after the great changes in the polarity of different parts is also a difference.二.Characteristics of system components multiflorum Thunb Pharmacodynamic Study groupObjective:Beans of different origin high-pressure system and polar parts of Polygonum multiflorum Polygonum multiflorum rat model of the role of blood and serum containing Polygonum multiflorum of granulocyte-macrophage hematopoietic progenitor cell line rat colony of the impact of cloning.Method:①female SD rats were randomly grouped,each 6-based model of the control group,normal control group and drug group,subjects in each drug group to a group of drugs.②rat model of blood deficiency.③in experiment 1,the beginning of the first oral drug delivery,for 9 consecutive days,day 1,dose 1ml/100g;in model group and normal control group given water cooler 1ml/100g.④1h after the last medication of heart blood,blood test.Result:1.Deqing high pressure multiflorum Thunb soy RBC,HGB,HCT indicators were higher than other high-pressure origin soy multiflorum Thunb,and its role in producing high-pressure better than other soy multiflorum Thunb,but compared with the normal group,the improvement will have limited impact. Polygonum multiflorum system in rat blood model for the improvement of a certain role,in which parts of the system n-butanol multiflorum Thunb the biggest difference.2.Multiflorum Thunb-containing serum on granulocyte-macrophage hematopoietic progenitor cell line cloned colony there is no significant impact.Conclusions:Origin beans from different high-pressure system multiflorum Thunb the routine treatment group indicators,high pressure Deqing multiflorum Thunb soy model for the blood of rats and some improvement,its role in producing high-pressure better than other soy multiflorum Thunb,but with the normal group compared to the limited role of the improvement.Polarity from different parts of the routine treatment group indicators,Health Shouwu rat model did not improve the role of blood deficiency,Polygonum multiflorum system parts in addition to chloroform,the remaining parts of the rat model are to improve the role of blood.By comparing the Health Shouwu and polar parts of the same system found in Polygonum multiflorum,the same difference in the size of the order of parts of n-butanol>ethyl acetate>full medicine> trichloromethane,N-butanol part of the biggest differences.Therefore,the choice of n-butanol part of the system comparison of Health and Polygonum multiflorum Shouwu difference map,we can identify the characteristics of the system components of the role of Polygonum multiflorum blood group.