The Effect Of Hypertonic Saline On Function Of Intestinal Mucosa Barrier In Rats With Acute Hepatic Failure

Objective:To establish the model of Acute liver failure in rats with induced intestinal barrier damage,and to study the impact of 7.5%hypertonic saline treatment on the protective role of intestinal barrier.Methods:46 SD rats were randomly divided into four groups:normal control(NC,A group),acute liver failure(ALF,B group),acute liver failure treated with 0.9%saline(ALF + NS,C group),and acute liver failure treated with 7.5%hypertonic saline(ALF + HTS,D group).The ALF Rat model(B,C,and D groups) was created by repeatedly treatment of 400mg/kg body weight thioacetamide(TAA) by intraperitoneal injections at 0 and 24 hours.Rats in C and D groups were treated with tail vein injection,4 ml / kg body weight,of 0.9%normal saline and 7.5%hypertonic saline respectively,at -30 minutes,18h and 36h post TAA injections.Rats in each group were slaughtered at 48h and underwent portal vein endotoxin,ATL,AST,TBIL and D-lactic acid levels measurements.The bacterial cultures of ileum and mesenteric lymph nodes were also measured.Finally,the end-of-ileum and liver were HE stained and ICAM-1 immuno-histochemstry staining analyzed.Results:1.The comparisons between B,C,and D group to A group show that: liver cell injury and the degree of intestinal mucosal injury was significantly higher(P＜0.01),ileum ICAM-1 expression was significantly enhanced;the number of intestinal bacillus and enterococcus were significantly increased whereas Lactobacillus and bifidobacteria was decreased significantly.The positive rates of bacterial detection in intestinal lymph node were at 100%,90%,and 54.55%for B,C and D groups respectively(10%was usually detected in A group).Finally,blood endotoxin,ATL, AST,TBIL and D-lactic acid levels were significantly higher(P＜0.01).2.The comparison between B and C groups revealed no significant differences (P＞0.05).3.In the D group,the comparisons with B and C revealed the following observations:Liver cell necrosis and the extent of intestinal mucous injury were reduced.The increasing of intestinal bacillus and enterococcus and decreasing of lactobacillus and bifidobacteria was not as obvious as in B,C groups.The bacteria detection rate in mesentery lymph node was reduces and the Endotoxin,ATL,AST, TBIL and D-lactic acid levels were decreased significantly;Lastly,the ileum ICAM-1 expression was lower.Conclusion:The injury of intestinal mucosa barrier caused by acute hepatic failure resulted in increased dysbacteriosis of intestinal,enhanced permeability,as well as bacterial translocation and endotoxemia.The application of 7.5%hypertonic saline showed some protective effect on gut barrier,thereby improving all those situations and slowing down liver and intestine pathological damage.