Study On The Separation And Identification & The Qualitity Control About The Effective Ingredient In Flemingia Philippinensis's

Flemingia macrophylla is Leguminous plants of Flemingia Roxb.ex Benth for the extraction with the root of Flemingia philippinensis Merr.The first time study on Flemingia Philippinensis for its active component to the separation,determination and identification of chemical structure.The first time purified from the two kinds of medicinal herbs monomer,and one kind of medicinal herb in a single quantitative study,using NMR analysis to determine the structure of compounds.For the Flemingia Philippinensis study and further development and utilization of its resources for the benefit of providing a scientific basis for humanity.一,Flemingia Philippinensis in the active component of the separation:Active ingredient from Flemingia Philippinensis was extracted by traditionary extraction method for Chinese Herbal using alcohol,and then the use of macroporous resin D101 type of medicinal herbs in the total flavonoids were isolated by quantitative analysis,and ultimately collect the highest total flavonoid content sub-stream that is 70%ethanol washing of the sub-stream.And then through silica gel column chromatography of total flavonoids in the main active component,through repeated column chromatography to be two types of monomers.At the same time tracking the use of thin-layer chromatography,carried out with three different doses were carried out on the monomer,the results are a single point,the two components of the initial monomer compounds targeting.Named componentâ…¡,componentâ…¢.二,Determination of active component:Using rutin as a relative standard for the use of ultraviolet spectrophotometry of total flavonoids Flemingia macrophylla quantitative research,results showed that the total flavonoid content reached 50%,in line with the five types of Chinese medicine for the standard.The use of high-performance liquid chromatographyâ…¢of components in the total content of flavonoids in the study,it has been reported in the literature flavonoids liquid phase separation conditions are as follows:methanol and water mixture;such as methanol and acetic acid mixture,the experimental use of the above mobile the effect of phase separation is not ideal,the more serious tailing.Therefore,to determine the final adjustment of the mobile phase methanol:0.02%phosphoric acid = 60:40,tailing phenomenon to be resolved and the total flavonoid fractionâ…¢be in a better separation.The results showed that the total flavonoid fractionâ…¢content of 1.09 percent.Through the methodology to verify that this method is simple,reliable and can serve as a single compound component method for the determination ofâ…¢.三,The structure of compounds identified:NMR instruments used to identify components of the monomer compoundsâ…¡,â…¢for structural identification of components.Soluble fractionâ…¢six deuterated dimethyl sulfoxide (DMSO-d6),in the measured hydrogen NMR spectrum(¹H-NMR),the whole spectrum of decoupling of carbon(¹³C-NMR),four methyl silicone n-(TMS) as internal standard.â…¡soluble components in deuterated chloroform,using the same tetramethyl silane(TMS) as internal standard by NMR(¹H-NMR) and the whole spectrum of decoupling of carbon (¹³C-NMR).Spectra through the solution for componentâ…¢genistein,Compositionâ…¡forβ-sitosterol.Research shows that Flemingia Philippinensis for the treatment of rheumatoid bone pain, lumbar muscle strain,chronic nephritis,bruises,carbuncle swelling,paralysis,impotence,and more women leucorrhea,prevention and treatment are widely used in gynecology.The main site for medicinal herbs in the total flavonoids.The first time in this study Flemingia Philippinensis in the two compounds were purified isoflavones,including genistein than the specific pharmacological effects,with some innovative.Reported in the literature at home and abroad genistein significantly the pharmacological effects,including the following aspects:一,Prevention and treatment of osteoporosis:genistein increased bone mineral density,the ability of estrogen receptor-binding,play a micro - Weak estrogenic effects,have the effect of bone mineral density increased,growth in tibia length,bone cells to form more than degradation to prevent bone loss,such as pharmacological effects,may become the treatment of osteoporosis new drug and efficient.二,Anti-tumor:Sanjeev Banerjee,genistein can be made to increase gemcitabine in vivo and in vitro anti-tumor activity in pancreatic cancer.Sharon A.,etc.on human breast cancer cells were studied,rather than the spread of metastasis in primary breast cancer tumor burden is the main reason for the transfer,after genistein diet intervention often can reduce the transfer of digital breast cancer model.三,HypolipidemicGenistein on TNT WR1339-induced hyperlipidemia in rats,and reduce the serum cholesterol and the role of glycerol resin acid,which is particularly significant.å››,The role of estrogen and anti-estrogen effect:Experiments in mice show that the uterine weight gain 25mg genistein need 8mg, compared with estrone,the role of intensity is very small,the relative intensity of only 1:6900. Mechanisms:a low concentration of genistein competition with estrogen of estrogen receptor, thereby interfere with function of estrogen.五,Antioxidant:Huachen Wei,Will be implanted DNA genistein,the change in the integrity of the structure of DNA,interference with the selection of the type of oxidation,reducing the amount of ultraviolet radiation caused by the formation of 8-OHdG,which played the role of antioxidant.å…­,Anti-hepatic fibrosis in vitro:Such as Qi Lihong Observed the expression of genistein on platelet-derived growth factor PDGF and the secretion of transforming growth factor TGF-β1 to promote hepatic stellate HSC-T6 cell proliferation and collagen synthesis and collagen typeâ… expression. Genistein can significantly inhibit or block cytokines fibrosisPDGF and TGF-β1 on the role of hepatic stellate cells,with anti-hepatic fibrosis in vitro,and its clinical application needs further study. At home and abroad have been approved with a wide range of master of the isoflavone genistein products as food for the crowd.U.S.National Cancer Research Center in 1996, genistein has been included in clinical chemoprevention of tumor development plans,the main objective is breast cancer and prostate cancer.At the same time,the United States in 1998 approved by SFDA in two main containing genistein(150mg / tablets) capsules for the food crowd.Female beauty can also be used for other health and the prevention of blood diseases.Genistein as phytoestrogens,are widely used in medicine,health products market.At home and abroad of its pharmacology,toxicology,structural modification of the study are to a certain extent,but it remained to study in depth its bioavailability be further raised.This study for the opening up of new medicinal Flemingia Philippinensis in some areas and theoretical basis of reference;Flemingia Philippinensis active role on the further development of in-depth study and also has some reference value.