Studies On Chemical Constituents Of The Roots Of Indigofera Kirilowii

The genus Indigofera comprises about 700 species widely distributed in the tropical and subtropical regions of the world, and in China it is represented by 80 species. The roots of several Indigofera species are used as substitutes for traditional Chinese medicine"ShanDouGen"(Sophora tonkinensis Gapnep.) in many provinces of China. They are most commonly used in the treatment of inflammatory diseases, especially sore throat, while it has also been reported that some patients exhibited neural poisoning symptoms after taking high dosages.In this thesis, we studied the chemical constituents of the roots of I. Kirilowii, which is one of the main resources of so-called"MuLanShanDouGen". Eighteen compounds were isolated and purified from the alcohol extracts by solvent extraction, silica gel column chromatography, macroporous resin column chromatography and re-crystallization. On the basis of various modern spectral analysis and classical chemical methods, the structures of sixteen compounds were elucidated as ethyl linoleate (IKrS-1), sitosterol linoleate (IKrS-3), long-chain n-alkyl caffeate (IKrS-4), lupeol (IKrS-5), sitosterol (IKrS-6), betulinic acid (IKrS-7), 1-monopalmitin (IKrS-8), 3-acetyloleanolic acid (IKrS-9), 6-O-(3-nitropropanoyl)-α,β-D-glucopyranose (IKrE-11), Ononin (IKrE-12), 3,4-di-O-(3-nitropropanoyl)-α,β-D-glucopyranose (IKrE-13), 3-nitropropanoyl acid (IKrB30-1), 2,6-di-O-(3-nitropropanoyl)-α-D- glucopyranose (IKrB30-2), glucosyringic acid (IKrB30-3), 2,3,6-tri-O-(3- nitropropanoyl)-α-D-glucopyranose (IKrB50-1), hesperidin (IKrB50-2). Among these compounds, IKrE-13 is a new natural product, IKrS-1, IKrS-3, IKrS-4, IKrS-8, IKrS-9, IKrE-11, IKrE-12 and IKrB50-2 were isolated From the title plant for the first time. IKrS-2 is one of the long-chain aliphatic acids, and IKrB70-1 is being identified. IKrE-11, IKrE-13, IKrB30-1, IKrB30-2 and IKrB50-1 could be responsible for the toxicity of the roots of I. kirilowii.We also summarized the studies on chemical constituents and bioactivities of Indigofera. Meanwhile, the characteristics of 3-nitropropanoyl glucopyranoses were summarized, too.