Studies On The Chemical Constituents Of The Roots Of Indigofera Carlesii Craib(Leguminosae)

The roots of Indigofera spp have been used as main substitutes for traditional Chinese drug"ShanDouGen"in many provinces of China such as Shanxi, Henan,Hubei, Anhui and so on, usually called"Mu-Lan-Shan-Dou-Gen"or"Tu-Dou-Gen". In this thesis, we studied the chemical constitutes of the roots of Indigofera carlesii Craib (Leguminosae) which is one of the main resources of"Mu-Lan-Shan-Dou-Gen". Nine Compounds were isolated and separated from the EtOAc and n-BuOH extracts of the roots of Indigofera carlesii Craib (Leguminosae) by macroporous resinD-101,solvent extraction, silica gel column chromatography and re-crystallization, and six known compounds were identified by various modern spectral analysis ( 1HNMR, 13CNMR, DEPT, 1H-1HCOSY, HSQC, HMBC, TOCSY, NOESY ) and classical chemical methods, Their structures were elucidated as Friedelin(ICP-Y1), 2,3,6-tri-O-[3-nitropropanoyl]-α-D-glucopyranose(corollin, ICB30-G1), n-Butyl-β-D-Fructofuranoside(ICB30-G2), 3-nitropropionic acid( ICB30-G6 ) , 2,6-di-O-[3-nitropropanoyl]-α-D-glucopyranose ( coronarian ,ICB30-G7), 3-O-α-L- rhamnopyranosyl(1→2)-[β- D–galactopyranose(1→3)] -α-L-arabinopyranosyl oleanolic acid 28-O-α-L- rhamnopyranosyl(1→4)-β- D–glucopyranosyl(1→6)-β- D–glucopyranosyl ester(ICB50-G4).Compounds ICB50-G4, ICB30-G2, ICP-Y1 were first isolated from I. carlesii, and compounds ICB50-G4 were obtained from Leguminosae for the first time. Compounds ICB30-G1, ICB30-G6, ICB30-G7 could be responssible for the toxicity of I. Carlesii Craib.In this thesis, we also summarized the studies on botanical resources, chemical constitutes and bioactivities of Indigofera species.