Clinical Research And Empirical Study Of Combination Treatment With Gemcitabine Chemotherapy And Huai'er Granula On Pancreatic Cancer

1.Experiment Study of the Influence on Growth and Apoptosis induced by Extract of Fungi of Huai'er in Human Pancreatic Cancer Cell Line Panc-1Objective: To investigate the growth inhibition and apoptosis induced by Extract of Fungi of Huai'er in human pancreatic cancer cell line Panc-1.Methods: Extract of fungi of Huai'er with different concentration gradient were cultured with human pancreatic cancer cell line Panc-1. Cell growth was assessed by MTT and FCM showed the change of cell apoptosis rate.Results: Extract of fungi of Huai'er induced a dose-dependent inhibition of human pancreatic cancer cell line Panc-1 in vitro. The maximum percentage of growth inhibition was17.7% at a concentration of 3mg/ml. The apoptotic ratio of human pancreatic cancer cell line Panc-1 increased parallelling with the increase of concentration of extract of fungi of Huai'er When the extract of fungi of Huai'er concentration was 3mg/ml, the apoptotic ratio reached (13.17±1.20)%, which was higher then that of group 6mg/ml(11.21±0.87)%, (P<0.05).Conclusions: Extract of fungi of Huai'er can significantly inhibit cell growth and induce apoptosis of human pancreatic cancer cell line Panc-1 in vitro, the optimal concentration is 3mg/ml.2. Clinical Research of Combination Treatment with Gemcitabine Chemotherapy and Huai'er Granula on Pancreatic CancerObjective: To study the clinical effect of Combination Treatment with gemcitabine(GEM) and Huai'er Granula on pancreatic cancer.Methods: In this prospective randomized controlled study, select 151 cases of pancreatic cancer(66 cases have undergone pancreectomy, 85 cases weren't resected)during Jul. 2004 to Sep. 2006, which included gemcitabine chemotherapy [33 cases (pancreectomy), group a + 42 cases (weren't resected), group A], gemcitabine+Huai'er Granula [33 cases (pancreectomy), group b + 43 cases (weren't resected), group B] and plus blank group [17 cases (pancreectomy), group c + 23 cases (weren't resected), group C], compared clinic effect of each group.Results: The blood side-effect of group GEM and GEM+Huai'er were 76.32% and 61.84% after treatment, the difference was significance (P<0.05). After chemotherapy, the group GEM+ Huai'er of the levels of IgG and IgM, the percentage of Nk cells and CD3+, rate of CD4+/CD8+ were higher than group GEM, the difference were significance (P<0.05). The rates of clinical benefit response of group GEM , GEM+ Huai'er were higher than the blank group(P<0.05), on the 3rd month, the difference of group GEM+ Huai'er compared GEM was no significance (P>0.05); on the 6th month, the difference of group GEM+ Huai'er were higher than group GEM (P<0.05). The rates of objective tumor response of group A, B were higher than group C(P<0.05), but the difference between group B and A was no significance (P>0.05). The recurrence and metaptosis rate of group a, b and c have no significant difference(P>0.05). there were no significant difference between GEM chemotherapy and GEM+Huai'er combined therapy in 1 year survival rate (P>0.05).Conclusions: Treatment of pancreatic cancer with gemcitabine chemotherapy combined Huai'er Granula improved the rates of objective tumor response , recurrence and metaptosis and survival rates of 1 year didn't have statistical evidence, but it improved side-effect of chemotherapy, survival quality and immune function.