The Effect Of Aromatics Herbs On Expression Of Aquaporin-4 MRNA In Cultured Astrocytes

The AQPs are a family of homologous water channel proteins(number at least 1 3 in mammals that provide the major route for water movement across plasma membranes in a variety of cell types and play a key role in maintain the water balance in brain and the microenvironment in cell.Aquaporin-4(AQP4)is the major water channel in the CNS , Its expression at fluid-tissue barrier (blood-brain and brain-cerebrospinal fluid barriers)throughout the brain and spinal cord suggests a role in water transport under normal and pathological conditions AQP4.Further insight into the mechanisms by which brain AQPs are regulated will be of utmost clinical importance,since perturbed water flow via brain AQPs has been implicated in many neurological disease。Objective To study the mechanism concerning herbs of resuscitation with aromatics on treating central nerve disease. We investigated the effect of drug-containing cerebrospinal fluid which extracted from rabbits on the expression of aquaporin(AQP) mRNA in primary cultured brain astrocytes (AST) of mice.Methods We stimulate primary cultured brain AST with cerebrospinal fluid drawning out from rabbits treated by intragastric administration of musk solution, solution of volatile oil from acorus tatarinowii, then examine the expression level of AQP4 mRNA by RT-PCR. Results AQP4 mRNA was expressed in the primary cultured brain AST. There was no influence on the expression of AQP4 mRNA when added control cerebrospinal fluid to culture medium. The cerebrospinal fluid contained calamus depress the expression of AQP4 mRNA. However the cerebrospinal fluid contained musk have no remarkable effect on the expression of AQP4 mRNA. Conclusion Valitile oil from acorus tatarinowii may decrease the expression of AQP4 mRNA .