Study On The Extraction And Pharmacodynamics Of Solanum Lyratum Thunb Polysaccharides

(Background]The Chinese herbs medicinal has the characteristics of lower poisonous and side effect, lower drug residue and difficultly making bacteria tolerant to the drug. Our country possesses the greatest resources of the Chinese herbs medicinal , but plenty of them are waiting to be developed, therefore reasonably developing the resources of the Chinese herbs medicinal is important and has promising future. At present, more and more researcher study on the Chinese herbs medicinal at home and abroad. Research results indicate that Solanum Lyratum Thunb has remarkable antibacterial and antiviral effect and can enhance immunity. But the research of Solanum lyratum Thunb Polysaccharides was not enough, there was no report about the Solanum Lyratum Thunb Polysaccharides content measuring and study from gene level about mechanism of enhancing immunity until now.[Objects](1)To study the extraction technology of Solanum Lyratum Thunb Polysaccharides.(2) To measure content of Solanum Lyratum Thunb Polysaccharides.(3)To measure antibacterial effect of Solanum Lyratum Thunb Polysaccharides.(4) To investigate the effects of Solanum lyratum Thunb Polysaccharides on mRNA expression of some cytokines in mice.[Methods](1) The polysaccharides was extracted from Solanum lyratum Thunb and its content was measured by phenol-sulfuric method .(2) Antibacterial effect of Solanum Lyratum Thunb polysaccharides was studied by cup-plate method and minimal inhibitory concentration (MIC) of Solanum Lyratum Thunb polysaccharides was measured by double dilution tests.(3) Balb/c mice were divided into four groups: group of control that were injected normal saline(C);group that were injected high(H),moderate(M),low(L) dosage Solanum Lyratum Thunb polysaccharides respectively. The four groups were administered for seven days. The mRNA expression level of IL-2,IL-12 and TNF- α were examined by RT-PCR at the 8th day.[Results]...