The Efficacy Of Material Basis For Drag's Blood Modulates Wide-dynamic Range Neuron Evoked Discharge In The Spinal Dorsal Horn Of Rats

Pain is endangering human health one of the major problems. In the early 21st century, As the pain gradually in-depth study, Researching and developing with high efficiency, without the side effects of new analgesic drug has become today's clinical research and drug hot spots. Our laboratory, based on traditional medicines dragon's blood analgesic effect of documentation, clinical observations have found that dragon's blood analgesic effect of the chemical composition of developing into a very safe and effective, low addiction potential new analgesic drugs. For these reasons, we use patch-clamp experiments, observing and confirming the analgesic effect of dragon's blood have one of the mechanisms that direct intervention by the conduction of pain information. At the same time, via in vitro experiments and mathematical theories analysis make certain of the people have been unable illustrates efficacy of chinese medicine-material definition. However, only through patch clamp experimental technology of dragon's blood in vitro can not be fully shows that all the pharmacological effects of the analgesic mechanism and its material basis for efficacy.Therefore, to confirm the material basis for efficacty of modulation of drgaon's blood on the evoked discharge of the wide dynamic rage neuron in spinal dorsal horn of rats with electrical stimulation sciatic nerve, using single extarcellular recording on intact male wister rat, the effect of three components cochinchinenin A, cochinchinenin B, loureirin B and these three kinds of chemical components in accordance with their dragon's blood in the percentage of contents in the form of various combinations on the evoked discharge of the wide dynamic range neuron in spinal dorsal horn of rats with electrical stimulation sciatic nerve were observed. In describing drug dose-response curve of the Hill coefficient of different circumstances, using the concept of dose equivalence, we developed a new zero interaction isobologram modeling for evaluation of triple drugs. At last, we make use of triple durgs isobologram modeling and binary durgs isobologram modeling that introduced by Tallarida to confirm the interaction of three cheminal components when they combined modulation the evoked discharge of the wide dynamic range neuron in spinal dorsal horn.The investigation demonstrated that dragon's blood suppressed the wide dynamic range neuron evoked discharge frequency with electrical stimulation sciatic nerve in a dose-dependent way, and achieved the greatest effect, the Hill coefficient of dose-dependent curve were different. With Three kinds of chemical components in various combinations, only the effect of the combination of cochinchinenin A, cochinchinenin B, and loureirin B were in good agreement with those of dragon's blood. The concentrations of the three components used alone were respectively higher than those used in combination when the same.The combined effects of the three components were synergistic,whereas the combined effects of cochinchinenin A and cochinchinenin B, cochinchinenin A and loureirin B, cochinchinenin B and loureirin B were antagonism.These results suggested that the material basis for efficacy of dragon's blood modulation of primary sensory neurons sodium channel is also can interfere pain message transmission in the level of the spinal cord, also may explain some of the analgesic effect of dragon's blood. The three components with synergistic combination effects not only modulation the TTX-R sodium currents in DRG neurons but also suppressed the evoked discharge frequency in WDR neurons in spinal cord are further confirmed that the material basis for the efficacy dragon's blood is the combination of cochinchinenin A, cochinchinenin B, and loureirin B.