The Study Of The Effect Of Helicobacter Pylori And Expression Of HTERT, HMSH2 And P27 In Gastric Carcinoma And Its Significance

Aims To investigate the role and pathogenic mechanisms of telomerase and related factors such as Helicobacter pylori in the development of gastric cancer with histological techniques and a number of molecular biological methods.Methods Detecting the expression of hTERT,hMSH2 and p27 with an immunohistological method in the cancerous tissue of 93 patients with gastric cancer obtained during surgical operation.The normal tissue of the corresponding patients(at least 5 cm away from the cancer lesion) was used as a control.Results The positive rate of hTERT in the gastric cancerous lesions was 79.6%,which is significantly higher than that in the neighboring normal mucosa(22.6%)(P＜0.01);The rate in the Hp positive patients was higher than the Hp negative patients(P＜0.01) The positive rate of hMSH2 in the gastric cancerous lesions was 65.6%,which is significantly higher than that in the neighboring normal mucosa(38.7%)(P＜0.01);The rate in the Hp positive patients was lower than the Hp negative patients(P＜0.05).The positive rate of p27 in the gastric cancerous lesions was 46.2%,which is significantly lower than that in the neighboring normal mucosa(80.6%)(P＜0.01);The rate in the Hp positive patients was not statistically lower than the Hp negative patients(P＞0.05). Conclusions:1.The down-regulation of p27 and Hp induced changes in the expression of telomerase and hMSH2 are associated with the development of gastric cancer.