The Viral Etiology Study Of Infantile Hepatitis Syndrome

Objective To explore the role of human cytomegalovirus(HCMV),Epstein-Barr virus(EBV),herpes simplex virus(HSV) and hepatitis virus including Hepatitis A virus (HAV),epatitis B virus(HBV) and Hepatitis C virus(HCV) infection in the infantile hepatitis syndrome(IHS).Methods anti-HAV IgM,anti-HCV IgM,specific antigen and antibiody of HBV, HCMV-IgM and EBV VCA-IgM were tested by enzyme-linked immunosorbent assay (ELISA) in 47 infants with IHS and 40 health infants.HCMV DNA in morning urine samples was tested by PCR.EBV DNA in nasopharyngeal secretions and PBMC was tested by PCR-Southern in two groups.RESULT①In all of 47 patients,The positive rate of HCMV DNA was 68.1%(32/47),HCMV DNA did not detected in all of 40 normal contrast urine samples.The positive rate of HCMV-DNA in the patient group was siganifitantly high than that of normal control group,the difference was significant(x~2=43.08,P＜0.01).The positive rate of HCMV-IgM was 44.7%(21/47),however,it was negative in all of 40 health infants.The positive rate of HCMV-IgM in the IHS was siganifitantly high than that in health infants,the difference was significant(x~2=23.56,P＜0.01).②The positive rate of EBV DNA in 47 patient's blood sample was 19.1%(9/47),and only one health infant was positive in blood samples,the positive rate was 2.5%.The positive rate of EBV DNA in the HIS groups was siganifitantly high than that in the normal control group, the difference was significant(x~2=4.365,P＜0.05).The positive rate of EBV DNA in 47 patients nasopharyngeal secretions was 34.0%(16/47),and only one health infant was positive in nasopharyngeal secretions,the positive rate was 2.5%(1/40).The positive rate of nasopharyngeal secretions EBV DNA in IHS group was siganifitantly high than that in the normal control group,the difference was significant(x~2=13.67,P＜0.01).The positive rate of VCA-IgM in IHS group was 14.9%(7/47),it was negative in all of 40 healthy children.The positive rate of VCA-IgM in IHS group was siganifitantly high than that in the healthy children,the difference was significant(x~2=4.62,P＜0.05).③One sample is positive of HBsAg and one sample is positive both HBsAg and HBeAg,the positive rate was 4.3%.④However,HSV-IgM,HAV-IgM and HCV-IgM were negative in all children of both groups.⑤Of all 47 patients,18 were cured,25 improved,2 unchanged,and 2 died.CONCLUSION HCMV infection was the main reason of HIS,and EBV infection has correlation with some HIS,but the percentage of IHS infected by HBV was obviously decreased.