Study On The Key Technology Of Quality Control And Quality Evaluation System Of Flos Lonicerae

Objective:Under the guidance of the theory and practice of Chinese medicine,made a systematic study began with textual criticism of Flos lonicerae and resources,species identification,evaluation methods and quality indicators,fingerprints,pesticide residues and heavy metals,as well as the quality of medicines related to Flos lonicerae.To identify and gradually establish a more complete,reliable,scientific evaluation of the quality of traditional Chinese medicines,establish a key technology of quality control and quality evaluation system for Flos lonicerae.To achieve a safe,effective,stable and controllable quality of Chinese medicine,reach the modernization and lay a good foundation to the world for traditional Chinese medicine.Methods:Connect with the quality control and fingerprints of the progress at home and abroad,the subjects using electrophoresis and RAPD markers to analysis DNA fingerprinting on two strains of Flos lonicerae at the molecular level for the cultivars provide a basis for classification and identification.Qualitative and Quantitative Analysis by HPLC were investigated from different sources and harvest time,processing methods from different areas,different storage time and the quality of Flos lonicerae related studies.Carry out routine examination of Flos lonicerae.Build HPLC fingerprint collection of illustrative plates.Structure the quality control Standard draft and draft explanation of Flos lonicerae.Results and conclusions:This subjects use RAPD markers for different varieties of Flos lonicerae conducted DNA fingerprint analysis for the first time,at the molecular level on the genetic marker studies,and provides a basis for Flos lonicerae species resource description,evaluation and identification.Verify the "Genuineness" ingredients advantages, optimize out the best optimum harvest period,the best processing methods and the best storage time for the construction of GAP data base support and technical support.Set up 16 common peaks of different origin Flos lonicerae fingerprints,and 19 common peaks of Shandong Flos lonicerae fingerprints.And for the first time successfully mark chlorogenic acid,caffeic acid,rutin,luteolin glycosides and quercetin five known peaks in HPLC fingerprint of Flos lonicerae.For the first time detect 666(total BHC),DDT(total DDT), pentachloronitrobenzene(PCNB) for the three kinds of pesticide content,and provide data to support the pesticide limits standards which the Pharmacopoeia defined to Flos lonicerae. Construct the quality evaluation system of Flos lonicerae finally.