Evaluation Of Quality Study Lonicerae Japonicae Flos Extract

Objective: Choosing Lonicerae Japonicae Flos,Lonicerae Flos,Eucommiae Folium,and yinhuang preparation series as the research object,to study the quality of Lonicerae Japonicae Flos extract,explore the specific methods of Lonicerae Japonicae Flos extract quality control,Lonicerae Japonicae Flos extract and compound preparat ions for the improvement of quality standards and testing methods to provide ideas.Methods: 1.A preliminary study: According to the Chinese pharm acopoeia2015 edition required to research and analyze the quality of the extracts from Flos Lo nicerae,select qualified medicine as the research object,establish Lonicerae Japonicae Flos extract the preliminary research on the quality control method,mainly through the Lonicerae Japonicae Flos,Lonicerae Flos,Eucommiae Folium of different regions of the original medicinal herbs extracts and eucommia ulmoides oliv commercial extr act content of chlorogenic acid in Flos Ionicerae and feature maps research and analyz the relationship between,and comparing with standard map Lonicerae Japonicae Flos extract;At the same time the quality of Lonicerae Japonicae Flos extract and yihuang preparations were analyzed,and thus find out the content change,the difference of composition and alternative rule,for the quality control of Chinese medicines,Lonice rae Japonicae Flos extract and provides a specific,accurate and reliable,fast compre hensive evalution method.2.Preparation research: Through the content of the bases of yi-huang granule,using HPLC gradient elution method under the same conditions and at the same time in the determination of yihuang granule of chlorogenic acid,caffic acid,rutin,mignon ette glycosides,content of baicalin,mignonette element,and use the analysis methods based on different manufacturer yi-huang granule content of the index components in comparison.Results:1.In different regions of Lonicerae Japonicae Flos,Lonicerae Flos,eucommia Folium,and original medicinal materials,extract and commercially available Lonicerae Japonicae Flos chlorogenic acid content in the extract,therelationship between the feature maps are studied,and the quality of Lonicerae Japonicae Flos extract and yihuang series preparations analysis showed that the origin al medicinal herbs and extracts are Lonicerae Flos contained chlorogenic acid content is the highest mountain;Lonicerae Japonicae Flos extract of eucommia ulmoides and chlorogenic acid content in the leaf extract has no obvious difference;Extract from Lonicerae Japonicae Flos,Lonicerae Flos,acidification barks of eucommia ulmoides oliv chlorogenic acid content increased significantly;Commercial extract content of chlorogenic acid in Flos Lonicerae exists obvious difference,the chromatogram can reach the standard extracts from Flos Lonicerae map of each characteristic peak dema nd,but the manufacturer of different extracts from Lonicerae Japonicae Flos character ristic peaks exist significant difference,and part of the extracts showed more impurity peak.Lonicerae flos,duzhong leafs and acidition extract's feature chromatogram are the same as the standard honeysuckle extract map.Some of yinhuang preparation has been checked the geniposidic acid content of duzhong leaf and the macrant Hoside B content of lonicerae flos.2.Chlorogenic acid,caffeic acid,rutin,mignonette glycosides,baicalin and mignonette six components within the scope of the investigation concentration,all had good linear relationship with the peak area,stability,precision and repeatability RSD were below 3.0%,the average recovery was 96%~99%.Baicalin content in the sampl es with different manufacturers silver yellow particles,there was no significant differen ce of chlorogenic acid,caffeic acid,rutin,mignonette glycosides,mignonette content differences.Conclusion: In experimental study showed that the extracts from Lonicerae Japon icae Flos impossibly to add Lonicerae Flos and Eucommiae Folium,Which needs to str engthen quality control,not only for chlorogenic acid content and green chlorogenicac id,chlorogenic acid,implicit chlorogenic acid,3,4-O-2 coffee acyl quinic acid,3,5-coffee acyl quinic acid,4,5-coffee acyl quinic acid six characteristic peak to measure the quality of Lonicerae Japonicae Flos extract,should further establish more specific quality standards to control the quality of Lonicerae Japonicae Flos extract and compou ond preparations,Lonicerae Japonicae Flos extract and compound preparations for theimprovement of quality standards and testing methods to provide new ideas.