The Purification And The Preliminary Study On Immunological Activity Of Velvetantler Polypeptide

Velvet is the vast majority of male deer species has not been a growth of the ossification of David Kok, as Chinese medicine in China has 2,000 years of history, Its medicinal value in the practice of their predecessors are also gradually been fully confirmed. In China's traditional medicine, velvet and ginseng, along with a fungus Shangpin of Chinese medicine, Chinese medicine in clinical occupies an important position. Velvet as a special cartilage, is a rich variety of natural factors of the cytokines, in their process of self-growth, or bone, blood, nerve growth, cells, epithelial cell growth in tandem with a deer Of the most active growth point in his store before the ossification of a large number of growth factors, they are velvet clinical biochemistry role of the foundation of modern medical research showed that velvet with a wide range of pharmacological activity Velvet is all mammals in the most rapid growth of renewable organs, in a short period of time to prompt the growth and development of bone, blood vessels, skin, hair and nerves and other organs. Velvet to promote growth, promoting metabolism, anti-inflammatory and immune regulation, brain puzzle, anti-aging, health blood, and promote the healing of wounds and fractures pharmacological effect is known by the chemical composition can not be explained. Therefore, the velvet-rich control the growth of various organs of the various multiple types of cell growth factor is very meaningful.This study observed the velvet peptide in mice blood serum IL-2 content, spleen T-lymphocyte proliferation, spleen NK cell activity and peritoneal macrophage phagocytosis of the impact and tried to measure the immune velvet peptide Active role. The results showed that componentsⅢ,Ⅳand mixed-peptide group can significantly improve blood in mice IL-2 level, in vivo applications can significantly stimulate synergies ConA spleen lymphocyte proliferation and can significantly improve the mouse spleen NK cells The killing activity, and reached a very significant difference in the level of (p <0.01), is the most active componentⅣ, but components and componentⅣⅢand extracts no significant difference between the (p> 0.05); Through peritoneal macrophage phagocytosis neutral red experiment tells us that componentsⅢ,Ⅳand extracts can significantly improve mouse peritoneal macrophage phagocytosis (p <0.01), is the most active extracts, but extracts And components ofⅣⅢand there was no significant difference between the (p> 0.05); velvet peptide in mice immune function in the six experimental results, we found a dose relationship, with the concentration can increase and strengthen, but The difference was not significant.Conclusion: The results show that componentⅢandⅣand mixed peptide group can significantly increase the mice's immune cell function.