Research Of Biochemiccal Marker In The Patients With Acute Cerebral Hemorrhage

Objective:To study the correlation between the content of serum glial fibrillary acidic protein(GFAP),S10013,acrolein,polyamine oxidase(PAO)and the volume of bleeding,the areas of cerebral edema,the bleeding part as well as the prognosis in the patients with acute cerebral hemorrhageMethods:99 patients with acute cerebral hemorrhage and 50 normal controls were recruited from Shandong provincial hospital,the cerebral hemorrhage group were chosen after the symptom happens in 24 hours.Checked by CT or MRI on morbidity and 3 day after then and Calculating ICH and perihematomal edema volumes according to CT or MRI.The ICH volume on addsion is incipient hemorrhage and the ICH volume at 3 day is the final hemorrhage,there are 31 cases in enlarge group judged by the volume of hemorrhage.The blood sample was obtained in the first day,the third day,the seventh day,the fourteenth day after disease onset,ELISA was used to detected the serum GFAP and S10013,HPLC was used to detected the acrolein and polyamine oxidase(PAO).The volume of bleeding and the areas of cerebral edema were calculated by cranium CT.The nerve function deficiency score(NIHSS)was conducted in the day of getting blood.The modified Rankin score(MRS)was used to evaluated the nerve function turnover and the handicap situation.Results:(1)the content of serum S100βin cerebral hemorrhage group is significantly higher than that in control group in the first day after disease onset,the content went to climax in the third day,then it decreased slowly,in the fourteenth day it became normal.And the content of S100βcorrelated with the content of GFAP. (2)The content of serum GFAP in cerebral hemorrhage group significantly increased in the first day,it went to climax in the seventh day,it was still significantly higher than that in control group in the fourteenth day.(3)The content of serum PAO in general hemorrhage group significantly increased in the first day,then it decreased slowly,in the fourteenth day it has no differences with that in control group.The content of serum PAO in hematoma expanding group went to climax in the third day, it was still significantly higher than that in control group in the fourteenth day.(4)The content of serum acrolein in general hemorrhage group and hematoma expanding group significantly increased in the first day,they went to climax in the seventh day, they was still significantly higher than that in control group in the fourteenth day.(5) All the indexes had positive correlation with the volume of bleeding,and no significant correlation with the bleeding part.,All indexes had correlation with NIHSS and MRS.GFAP,PAO and Acrolein had correlation with the areas of cerebral edema.(6)The level of acrolein in the first day had significant correlation with hematoma expanding.Conclusions:(1)All the indexes increased,and had positive correlation with the volume of bleeding,which indicates that the four indexes can reflect damage degree of the brain tissue.(2)The content of serum GFAP,PAO,Acrolein could be used as clinical biomarker to judge the size of cerebral edema after cerebral hemorrhage.(3) The content of serum GFAP,PAO,S100βand Acrolein had significant correlation with the situation and prognosis of the patients with cerebral hemorrhage,and they are simple,cheap,effective blood pretest indexes for cerebral hemorrhage.(4)The content of serum arcolein could be used as clinical biomarker to judge the risk of hematoma expanding after cerebral hemorrhage.The content of serum PAO could be used as clinical biomarker to judge whether hematoma expanding after cerebral hemorrhage.