The Effect Of Atovastatin Preconditioning On Cerebral Neurospecific Enlase, Myelin Basic Protein And Glial Fibrillary Acidic Protein In A Rat Model Of Cerebral Ischemia Reperfusion

Objective:To investigate the effect of Atorvastatin (Ato) preconditioning on cerebral NSE, MBP and GFAP in a rat model of cerebral ischemia reperfusion.Methods:30 male SD rats were randomly assigned to cerebral ischemia/reperfusion group, Ato preconditioning group and sham group. The rats of Ato preconditioning group received Ato (5mg/kg.d) by gastric gavage for 5 consecutive days before molding while the other two groups received the same volume of saline. Transient focal cerebral ischemia was induced by 2 hours of middle cerebral artery occlusion (MCAO) and followed by 24 hours reperfusion. The neurological deficit were determined by Longa’s score. The expression of cerebral NSE, MBP and GFAP were measured with immunohistochemistry.Results:1. No neurologic impairment was observed in the rats of sham-operated group, while the NDS of Ato preconditioning group and control group were higher than sham-operated group; and the NDS of Ato preconditioning group were lower than control group (P<0.05); 2. Deeper GFAP staining cells were observed in Ato preconditioning group and control group than those in sham-operated group. The difference in AODs of GFAP staining cells between Ato preconditioning group and control group were of no significance(P>0.05).Conclusion:Atovastatin preconditioning improves neurologic impairment. Atorvastatin preconditioning prevents oligodendrocytes and neurons from cerebral ischemia reperfusion injury, and has little impact on astrocytes. Atovastatin preconditioning alleviates brain injury.