Fingerprints Of Yaotongning Capsule By HPLC-MS

Studying on HPLC-MS fingerprints of Yaotongning Capsule has been described in this thesis. The main content is as follows:The HPLC-MS fingerprint of Yaotongning Capsule was eatablished.The Yaotongning capsule was extracted by ultrasonic machine for 40 min, using methanol-H2O(50:50) as extracting solvent.Then the extracting solution was determined by RP-HPLC(MS) under following conditions: The chromatographic column is Aligent ZORBAX Eclipse XDB 250mm×2.1mm, 5μm,C18; The flow rate is 1.0ml·min-1;The mobile phase of the gradient elution mode was applied in chromatographic separation was:0.1% formic acid water (A)- 0.1% formic acid acetonitrile (B) (v/v):0min, 95%:5%; 15 min,90%:10%; 80min, 60%:40%; 120min, 40%:60%; 130min, 30%:70%; 150min, 0%:100%; 160 min, 0%:100%.The mass spectrometer parameters were as follows: utilizing electrospray ionisation (ESI), spray voltage 4.5KV, sheath gas(N2) flow rate 35 arb unit, auxiliary gas(N2) flow rate 5 arb unit, capillary temperature 300℃; The spectra was acquired in the positive ion mode and negative ion mode at the same time, full scan mass range m/z150-m/z1500, on line MS/MS.Only signals of at least 5% abundance that are entirely consistent and reproducible are reported here. An isolation breadth of 2u was used for the various MSn stages. The collision energy was kept at the instrument default value of 35%. The flow to the mass spectrometer was split by a Post-column, the ratio is 4:1.The chemical constituents reflected by the 43 chromatographic peaks in the HPLC -MS fingerprints of Yaotongning Capsule were affirmed and the corresponding medicinal materials ascriptions of these chemical constituents are analyzed; there are 15 chemical constituents were affirmed by analyzing the chemical constituents of Semen Strychni-the main medicinal material of Yaotongning Capsule.