Protective Effects Of Glucosamine And Chitooligosaccharide In Alcohol Liver Injury Mice

Alcohol Liver Disease is caused by continuous and excessive alcohol. It can reduce the power of eliminating O free radical, and make the liver fat. Besides, the alcohol can be transformed to acetaldehyde, which is harmful for liver. Alcohol liver injury can be divided into three kinds as alcohol fatter liver, alcohol hepatitis and alcohol hepatocirrhosis. The alcohol fatter liver is the mostly kind. When the fat cumulated in the liver excess 5% of the liver weight or we can see 1/3 of liver cells become fatty in histology, we can intitule it as alcohol fatter liver. Along with the improvement of standard of living and the change of life style, the sots increase everyday and there is a tendency of the increase of alcohol fatter liver. The alcohol fatter liver is a severest influence to the human and the society.Chitin which is the second most abundant biopolymer on the earth have been paid a great attention in many countries in many field as biology medicine, function food, light industry and environmental protection. Chito-oligosaceharide(COS) and the monomer-Glucosmaine(GlcNH2) have been widely used in many field as function food, animal feed additive and crops because its characteristic of low molecular weight, good water-solubility, good absorbability and avirulence. The protective effects of COS and GlcNH2 have not been thoroughly studied to our known besides a few reports on COS.In my study, the low molecular weight of chitin such as glucosamine and chitooligosaccharide are prepared. COS and GlcNH2 are purified and their biochemical parameters and physical properties are tested. We study the protective effect of COS and GlcNH2 in alcohol liver injury mice by body experiment. The rapid alcoholism model and alcohol liver injury model of mice are built by feeding alcohol continuously. The treated groups are fed Glucosamine or Chitooligosaccharide half an hour before feeding alcohol. The ALT, AST, GGT, TG, TC in the blood serum are measured after eight weeks, then fix up the liver and make pathologic sections and observe them by optical microscope.Our results from experiment showed: COS and GlcNH2 have obviously protective effects for rapid alcoholism mice. They can shot down the sober up time and reduce the death rate. They also have obviously protective effects for alcohol liver injury mice. The level of ALT, AST, GGT, TG, TC in model group are significantly increased compared with normal group (p<0.05). GlcNH2 and COS can effectively decrease all the five indexes near to normal level. The pathologic sections reflect there are many Mallory bodies in the liver of model group but in the groups fed by COS and GlcNH2, we can see less Mallory bodies.In a conclusion, COS and GlcNH2 have protective effects not only for rapid alcoholism mice but also alcohol liver injury mice. Our study provides some experimental basis for further study of the treatment of alcohol liver disease by COS and GlcNH2.